Identification and genetic diversity analysis of hybrid progeny of Clematis macropetala using ISSR markers
[Objective]This study was conducted to accurately and quickly determine the authenticity of the hybrid progeny of Clematis macropetala,to shorten the breeding time of new cultivars of C.macrop-etala,which is important for the early identification of the hybrid progeny and the breeding of new cultivars.[Method]In this study,30 progenies of 8 C.macropetala cross combinations were identified using 12 ISSR primers,and genetic diversity analysis was performed on the true hybrids and the parents.[Result]A total of 29 true hybrids were identified from 30 progenies,with a true hybrid rate of 96.67%.PCR amplification of the 29 true hybrids and 9 parents gave a total of 136 distinct bands,with a polymorphic band ratio of 99.26%.The average observed number of alleles(Na)was 1.992 6,the number of effective alleles(Ne)was 1.477 8,the genetic diversity of Nei(H)was 0.293 7 and Shannon's information index(I)was 0.453 1,indicating that 38 samples had rich genetic diversity.The genetic similarity coefficient matrix showed that 82.76%of the true hybrids were closely related to their male parents.[Conclusion]ISSR mo-lecular marker technology can accurately and quickly identify the authenticity of hybrid progeny of C.mac-ropetala.The genetic distance between progeny of the same cross combination is relatively close.Genetic differentiation has occurred in true hybrids and they have maintained a closer genetic relationship to their male parents.
NETL
NSTL
万方数据
维普
