盐胁迫对小麦籽粒萌发中淀粉分解及苗期根系生长的影响
Effect of salt stress on starch degradation in wheat seed germination and root system at seedling stage
朱妍钰 1汪军成 1姚立蓉 1孟亚雄 1李葆春 2杨轲 1司二静 1王化俊 1尚勋武 3马小乐1
作者信息
- 1. 省部共建干旱生境作物学国家重点实验室,甘肃省作物遗传改良与种质创新重点实验室,甘肃 兰州 730070;甘肃农业大学农学院,甘肃 兰州 730070
- 2. 省部共建干旱生境作物学国家重点实验室,甘肃省作物遗传改良与种质创新重点实验室,甘肃 兰州 730070
- 3. 甘肃农业大学农学院,甘肃 兰州 730070
- 折叠
摘要
[目的]通过测定盐(NaCl)胁迫下两个小麦品种籽粒萌发过程中的淀粉颗粒的分解、淀粉酶活性及根系生长等指标,研究并解释其耐盐性,以期为筛选耐盐小麦种质筛选提供理论依据.[方法]试验在小麦籽粒萌发过程中设0 mmol/L(CK)、100 mmol/L、200 mmol/L、300 mmol/L 4个NaCl胁迫浓度处理.在胁迫48 h时,使用扫描电子显微镜观察胚乳中淀粉颗粒的分布状况,利用3,5-二硝基水杨酸法测定籽粒中α-淀粉酶活性,发芽势及发芽率;在胁迫4、6、8、10、12 d时测定幼苗的根长、根面积、根体积等指标.[结果]与CK相比,随着NaCl浓度的增大,两个小麦品种籽粒胚乳A、B型淀粉粒的数量逐渐增多,紫小麦的A、B型淀粉降解情况优于甘春24号;两个小麦品种籽粒的α-淀粉酶的活性逐渐减小;在同一时期,两个小麦品种幼苗的发芽势、发芽率及根系生长指标均呈现逐渐减小趋势,且与CK差异显著(P<0.05),甘春24号降幅降低幅度高于紫小麦.[结论]紫小麦耐盐性优于甘春24号.
Abstract
[Objective]The salt tolerance of different wheat varieties was studied and explained by deter-mining the indexes of starch granule degradation,amylase activity and root growth during seed germination under salt(NaCl)stress,in order to provide a theoretical basis for screening salt-tolerant wheat germplasm.[Method]In this experiment,different concentrations of NaCl stress were applied during the seed germina-tion,and the stress concentrations were 0 mmol/L(CK),100 mmol/L,200 mmol/L and 300 mmol/L.The distribution of starch granules in the endosperm was observed under a scanning electron microscope after 48 h,and the α-amylase activity in the grains was determined by the 3,5-dinitrosalicylic acid method.Meanwhile,the germination potential,germination rate and the indicators such as root length,root area and root volume at 4 th,6 th,8 th,10 th and 12 th d of stress were measured.[Result]During the same period,the seedling germination potential,germination percentage and root growth index of the two wheat varieties showed a decreasing trend and were significantly different from CK(P<0.05).The decreasing amplitude of Ganchun 24 was lower than that of purple wheat.Compared with CK,the number of A-and B-type starch grains in the endosperm of the two wheat varieties gradually increased with the increasing NaCl concentra-tion.The activities of α-amylase of two wheat varieties gradually decreased.[Conclusion]The salt toler-ance of purple wheat was better than that of Ganchun 24.
关键词
小麦/NaCl胁迫/根系生长/淀粉分解Key words
wheat/NaCl stress/root growth/starch degradation引用本文复制引用
基金项目
甘肃省教育厅青年博士基金项目(2021QB-029)
甘肃省农业小麦产业体系项目(GARS-01-05)
省教育厅高校科研项目(2018D-14)
甘肃省科技重大专项计划项目(17ZD2NA016)
甘肃省科技计划项目(1506RJZA014)
出版年
2024
NETL
NSTL
万方数据