Effect of salt stress on starch degradation in wheat seed germination and root system at seedling stage
[Objective]The salt tolerance of different wheat varieties was studied and explained by deter-mining the indexes of starch granule degradation,amylase activity and root growth during seed germination under salt(NaCl)stress,in order to provide a theoretical basis for screening salt-tolerant wheat germplasm.[Method]In this experiment,different concentrations of NaCl stress were applied during the seed germina-tion,and the stress concentrations were 0 mmol/L(CK),100 mmol/L,200 mmol/L and 300 mmol/L.The distribution of starch granules in the endosperm was observed under a scanning electron microscope after 48 h,and the α-amylase activity in the grains was determined by the 3,5-dinitrosalicylic acid method.Meanwhile,the germination potential,germination rate and the indicators such as root length,root area and root volume at 4 th,6 th,8 th,10 th and 12 th d of stress were measured.[Result]During the same period,the seedling germination potential,germination percentage and root growth index of the two wheat varieties showed a decreasing trend and were significantly different from CK(P<0.05).The decreasing amplitude of Ganchun 24 was lower than that of purple wheat.Compared with CK,the number of A-and B-type starch grains in the endosperm of the two wheat varieties gradually increased with the increasing NaCl concentra-tion.The activities of α-amylase of two wheat varieties gradually decreased.[Conclusion]The salt toler-ance of purple wheat was better than that of Ganchun 24.
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