辣椒CaPME基因家族鉴定及表达分析
Identification and expression analysis of CaPME gene family in pepper
匡小妍 1段盼盼 1魏敏 1刘芳 1马玉虎 1马艳 1张涛 1魏兵强1
作者信息
- 1. 甘肃农业大学园艺学院,甘肃 兰州 730070
- 折叠
摘要
[目的]了解辣椒CaPME基因家族及其表达模式,为探索其在抵抗逆境过程中的作用,以及培育新型抗逆品种提供参考.[方法]采用生物信息学方法对CaPME基因进行鉴定分析.[结果]在辣椒中有64个CaPMEs基因家族成员,所有成员C末端均含保守结构域Pectinesterase,部分成员N末端含有PMEI结构域;CaPMEs基因的外显子数量为1～6个,基因大部分基序分布在N端,除了CaPME16、CaPME39和CaPME62,大部分基因基序长度在600个氨基酸范围内;分析顺式作用元件发现,在辣椒CaPMEs上游2000 bp序列中存在多种响应元件,包括光、激素、厌氧诱导、低温等响应元件;部分CaPMEs 家族成员在花药和子房中特异表达;部分基因在NaCl胁迫和冷胁迫下,具有特异性表达.[结果]鉴定出了64 个CaPMEs基因家族成员,部分基因在不同组织和非生物胁迫下存在特异表达.
Abstract
[Objective]This study aimed to provide a reference for further understanding the role of the CaPME(Capsicum Pectin methylesterase)gene family in stress resistance and the development of new stress-resistant pepper varieties by investigating its gene composition and expression patterns.[Method]Bioinformatics methods were employed to identify and analyze the CaPME gene family.[Result]The pep-per genome contained a total of 64 members belonging to the CaPME gene family,all of which possessed a conserved Pectinesterase domain at the C-terminal,and some members have a PMEI domain at the N-terminal.The number of exons in the CaPME genes ranged from 1 to 6,with the majority of the gene struc-tures located in the N-terminal region.Except for CaPME16,CaPME39,and CaPME62,most members had a length of approximately 600 amino acids.Analysis of cis-acting elements revealed the presence of nu-merous response elements within the 2 000 bp sequence upstream of CaPME genes in pepper,including light-responsive,hormone-responsive,anaerobic induction,and low temperature-responsive elements.Sev-eral members of the CaPME gene family exhibited differential expression in anthers and ovaries,while some genes showed specific expression patterns under NaCl and cold stress conditions.[Conclusion]In this study,a total of 64 members of the CaPME gene family were identified,and specific expression patterns were observed in different tissues and under various abiotic stress conditions.
关键词
辣椒/PME/基因家族/生物信息学/表达分析Key words
pepper/PME/gene family/bioinformatics/expression analysis引用本文复制引用
基金项目
甘肃省科技厅重点研发计划项目(21YF5NA091)
兰州市人才创新创业项目(2021-RC-65)
出版年
2024
NETL
NSTL
万方数据