Effect of potato dextrose agar medium(PDA)from different varieties(lines)on saprophytic fungi growth
[Objective]Fusarium oxysporum,Fusarium avenaceum,Fusarium solani and Fusarium tricinctum were used as test materials for the screening of potato species suitable for use as saprophytic fun-gal potato glucose medium(potato dex-trose agar,PDA).[Method]Fifteen different potato varieties(lines)were selected and commercial PDA medium was used as the control group to compare the growth rate and spore production of F.oxysporum mycelia on PDA medium made from different potato varieties;on this ba-sis,the suitability of the potato varieties(lines)for the growth of other Fusarium strains was further verified.[Result]The potato varieties(lines)17W-12 and Atlantic PDA media were the most effective in support-ing the growth rate and sporulation of F.oxysporum mycelium,and the mycelial density,uniformity and edge neatness were better than those of other varieties(lines).PDA medium of Longshu 7 and Qingshu 9 showed the worst performance.The growth rate and spore production of the four Fusarium species were sig-nificantly higher in the PDA medium of Atlantic than in the other treatment groups:compared with the PDA medium of Qingshu 9,cultured on Atlantic PDA medium for 7 days,the average mycelial growth rate of 4 Fusarium(F.oxysporum,F.avenaceum,F.solani and F.tricinctum)increased by 243.73%,243.75%,171.92%,and 126.28%,respectively.Sporulation yield increased 18.59 times,4.49 times,1.27 times and 2.19 times,respectively.Compared with commercial PDA medium,cultured on Atlantic PDA medium for 7 days,the average mycelial growth rate of 4 Fusarium(F.oxysporum,F.avenaceum,F.solani and F.tricinctum)increased by 74.53%,78.33%,178.60%,and 145.09%,respectively.The sporulation yield in-creased 6.57 times,2.14 times,24.64 times and 1.57 times,respectively.[Conclusion]Atlantic is a classic variety of processed potato widely cultivated around the world and can be used as a preferable raw material for PDA medium.
potato varieties(lines)mediumsaprophytic fungimycelial growth ratespore production
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