首页|独脚金内酯参与富氢水增强番茄幼苗根系的耐盐性

独脚金内酯参与富氢水增强番茄幼苗根系的耐盐性

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[目的]研究盐胁迫下氢气(H2)和独脚金内酯(SLs)在番茄幼苗根系中的调控作用及其相互关系.[方法]以番茄(Solanum lycopersicum L.'Micro-Tom')为试材,采用人工培养室基质栽培方式,确定最适浓度富氢水(75%)的基础上,设置如下处理:100%霍格兰营养液(CK)、盐胁迫(NaCl)、NaCl+HRW、NaCl+GR24(独脚金内酯合成类似物)、NaCl+HRW+TIS108(独脚金内酯合成抑制剂).测定根系相关指标以及SLs含量及其相关合成基因表达量.[结果]盐胁迫下,75%HRW处理能显著增加番茄根系的长度、鲜质量和干质量.同时,GR24和HRW促进了盐胁迫下番茄幼苗的根系形态参数(根长、根表面积、根体积、平均直径等)和根系活力.然而,HRW的积极作用被SLs合成抑制剂TIS108所抑制.所以H2调节的番茄幼苗根系耐盐性可能SLs与有关.此外,HRW处理增加了盐胁迫下番茄幼苗根系的内源SLs含量.在盐胁迫下,HRW处理也显著上调了SLs生物合成相关基因SlCCD7、SlCCD8、SlD14和SlMAX1的表达水平.[结论]SLs可能参与了H2诱导的番茄幼苗根系的耐盐性.
nvolvement of strigolactone in hydrogen-rich water enhanced salt tolerance in tomato seedling roots
[Objective]To investigate the regulatory roles of hydrogen(H2)and strigolactones(SLs)and their interaction in the roots of tomato seedlings under salt stress.[Method]Tomato(Solanum lycopersi-cum L.'Micro-Tom')was used as the experimental material,and was grown in an artificial culture cham-ber substrate.Based on the optimum concentration of hydrogen-rich water(75% ),the treatments are as fol-lows:100% Hogland(CK),salt stress(NaCl),NaCl+HRW,NaCl+GR24(SLs synthesis),NaCl+HRW+TIS108(SLs synthesis).Root related parameters,SLs content,and the expression of SLs biosyn-thesis related genes were determined.[Result]Under salt stress,75% HRW treatment significantly in-creased root length,fresh weight,and dry weight of tomato seedling roots.Meanwhile,GR24 and HRW pro-moted root morphological parameters(root length,root surface area,root volume,root/shoot ratio,etc.)and root activity of tomato seedlings under salt stress.However,the beneficial roles of HRW were blocked by TIS108(SLs inhibitor).Thus,SLs may be responsible for H2-regulated salt stress tolerance in the root of tomato seedlings.Additionally,HRW treatment increased endogenous SLs levels in tomato seedling roots under salt stress.HRW treatment significantly up-regulated the expression levels of SLs biosynthesis-related genes SlCCD7,SlCCD8,SlD14 and SlMAX1 under salt stress.[Conclusion]SLs may be involved in H2-induced salt tolerance in the root of tomato seedlings.

tomatosalt stresshydrogen rich waterstrigolactonesroot activitygene expression

叶福金、方华、冯丽、石媚媚、黄盼盼、刘兴娟、刘泽生、廖伟彪

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甘肃农业大学园艺学院,甘肃 兰州 730070

番茄 盐胁迫 富氢水 独脚金内酯 根系活力 基因表达

国家重点研发计划项目国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目中国博士后科学基金项目中国博士后科学基金项目甘肃省自然科学基金项目甘肃省自然科学基金项目甘肃省教育厅创新创业团队项目

2018YFD100080032072559318605683156056331160398201004708872012T508281606RJZA0731606RJZA0772018C-14

2024

10.13432/j.cnki.jgsau.2024.03.015

甘肃农业大学学报
甘肃农业大学

甘肃农业大学学报

CSTPCD北大核心
影响因子：0.612
ISSN：1003-4315
年,卷(期)：2024.59(3)