摘要
[目的]研究盐胁迫下氢气(H2)和独脚金内酯(SLs)在番茄幼苗根系中的调控作用及其相互关系.[方法]以番茄(Solanum lycopersicum L.'Micro-Tom')为试材,采用人工培养室基质栽培方式,确定最适浓度富氢水(75%)的基础上,设置如下处理:100%霍格兰营养液(CK)、盐胁迫(NaCl)、NaCl+HRW、NaCl+GR24(独脚金内酯合成类似物)、NaCl+HRW+TIS108(独脚金内酯合成抑制剂).测定根系相关指标以及SLs含量及其相关合成基因表达量.[结果]盐胁迫下,75%HRW处理能显著增加番茄根系的长度、鲜质量和干质量.同时,GR24和HRW促进了盐胁迫下番茄幼苗的根系形态参数(根长、根表面积、根体积、平均直径等)和根系活力.然而,HRW的积极作用被SLs合成抑制剂TIS108所抑制.所以H2调节的番茄幼苗根系耐盐性可能SLs与有关.此外,HRW处理增加了盐胁迫下番茄幼苗根系的内源SLs含量.在盐胁迫下,HRW处理也显著上调了SLs生物合成相关基因SlCCD7、SlCCD8、SlD14和SlMAX1的表达水平.[结论]SLs可能参与了H2诱导的番茄幼苗根系的耐盐性.
Abstract
[Objective]To investigate the regulatory roles of hydrogen(H2)and strigolactones(SLs)and their interaction in the roots of tomato seedlings under salt stress.[Method]Tomato(Solanum lycopersi-cum L.'Micro-Tom')was used as the experimental material,and was grown in an artificial culture cham-ber substrate.Based on the optimum concentration of hydrogen-rich water(75% ),the treatments are as fol-lows:100% Hogland(CK),salt stress(NaCl),NaCl+HRW,NaCl+GR24(SLs synthesis),NaCl+HRW+TIS108(SLs synthesis).Root related parameters,SLs content,and the expression of SLs biosyn-thesis related genes were determined.[Result]Under salt stress,75% HRW treatment significantly in-creased root length,fresh weight,and dry weight of tomato seedling roots.Meanwhile,GR24 and HRW pro-moted root morphological parameters(root length,root surface area,root volume,root/shoot ratio,etc.)and root activity of tomato seedlings under salt stress.However,the beneficial roles of HRW were blocked by TIS108(SLs inhibitor).Thus,SLs may be responsible for H2-regulated salt stress tolerance in the root of tomato seedlings.Additionally,HRW treatment increased endogenous SLs levels in tomato seedling roots under salt stress.HRW treatment significantly up-regulated the expression levels of SLs biosynthesis-related genes SlCCD7,SlCCD8,SlD14 and SlMAX1 under salt stress.[Conclusion]SLs may be involved in H2-induced salt tolerance in the root of tomato seedlings.
基金项目
国家重点研发计划项目(2018YFD1000800)
国家自然科学基金项目(32072559)
国家自然科学基金项目(31860568)
国家自然科学基金项目(31560563)
国家自然科学基金项目(31160398)
中国博士后科学基金项目(20100470887)
中国博士后科学基金项目(2012T50828)
甘肃省自然科学基金项目(1606RJZA073)
甘肃省自然科学基金项目(1606RJZA077)
甘肃省教育厅创新创业团队项目(2018C-14)
NETL
NSTL
万方数据