Physiological responses of Gymnocarpos przewalskii to simulated osmotic stress
[Objective]To study the response of Gymnocarpos przewalskii seedlings to PEG stress in order to provide a reference for elucidating the drought resistance mechanism of G.przewalskii.[Method]Under drought stress,the relative water content of the leaves of G.przewalskii seedlings decreased signifi-cantly.The ratio of bound to free water content first increased significantly and then decreased with increas-ing PEG concentration under 24 h stress.The content of proline and soluble sugars fluctuated under PEG stress and accumulated significantly under 24 h PEG stress.The content of malondialdehyde(MDA)in-creased with the increase of PEG stress and was significantly higher than that of the control under 24 h stress.Hydrogen peroxide levels increased significantly under PEG stress for 8 h and 24 h compared to the control.The activity of superoxide dismutase(SOD),catalase(CAT)and ascorbic acid peroxidase(APX)reached the significant level compared to the control under PEG stress for 8 h and 24 h,and the activity of peroxidase(POD)increased significantly under 24 h stress.[Conclusion]PEG stress for 24 h caused signifi-cant peroxidative damage to G.przewalskii seedlings.The main adaptation of G.przewalskii seedlings to osmotic stress was to increase the amount of bound water,to actively accumulate proline and dissolved sug-ars,and to increase the activity of SOD,POD,CAT and APX.
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