Optimal ratio of compound biopreservatives to the preservation effect of micro-frozen Micropterus salmoides
[Objective]To extend the shelf life of Micropterus salmoides,to provide a theoretical refer-ence for the storage and preservation of Micropterus salmoides.[Method]Potassium sorbate(PS),nisin(NI),pullulan(LP)and potassium Sorbate(PS)were selected for the single factor test.The response surface Box-Behnken test design was used to optimize the composute biological preservative.The optimum ratio was 0.22% NI,1.61% LP and 4.14% PS.pH value,total viable count(TVC)and total volatile base nitro-gen(TVB-N)of M.salmoides during micro-frozen storage were evaluated with the optimum ratio of the combined preservative.TVB-N value,actomyosin content,sulfhydryl content,Ca2+-ATPase activity and water holding capacity(WHC)were determined.[Result]At the end of the storage period(10 d),the pH of the combined biological preservative group(NLP group)was still below 7.0.The TVB-N value did not ex-ceed 12 mg/100 g;the total number of colonies did not exceed 5.0 lg(CFU/g).Water retention,actomyo-sin content,sulfhydryl content and Ca2+-ATPase activity decreased by 7.08%,11.01%,22.07% and 28.33%,respectively.At the end of the storage period,the pH of the control group(CK group)was higher than 7.0.The TVB-N value reached 12.55mg/100g;the total number of colonies exceeded 5.0 lg(CFU/g).Water retention,actomyosin content,sulfhydryl content and Ca2+-ATPase activity decreased by 10.75%,22.62%,34.78% and 40.48%,respectively.[Conclusion]The combined biological preservative could effectively slow down the changes in microbiological indexes and various physicochemical indexes of M.salmoides,and had a better effect on delaying fish deterioration and prolonging shelf life.
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