基于感官组学的烤烟烟叶中糯米香特征成分的鉴定
Identification of clutinous rice-like aroma components featured in flue-cured tobacco leaves based on sensomics approaches
李舒畅 1黄建 2庞夙 3李军 4刘贯山 5庞雪莉 5孔凡玉5
作者信息
- 1. 中国农业科学院烟草研究所,山东 青岛 266101;中国农业科学院研究生院,北京 100081
- 2. 江西中烟工业有限责任公司,江西 南昌 330096
- 3. 四川省烟草质量监督检测站,四川 成都 610041
- 4. 山东中烟工业有限责任公司,山东 济南 250014
- 5. 中国农业科学院烟草研究所,山东 青岛 266101
- 折叠
摘要
[目的]准确鉴定烤烟烟叶中糯米香关键特征成分.[方法]以典型糯米香香韵特征烤烟烟叶为研究对象,利用现代分子感官科学技术,借助气相色谱嗅觉检测/稀释嗅闻技术(GC-O/AEDA)以及多维气相色谱-高分辨质谱联用技术(GC×GC-QTOF/MS),对其糯米香关键特征成分进行了筛选和精确鉴定.[结果]利用该技术方法,从糯米香特征烤烟样品中筛选定位到一种具有气味强度高、检测频率高、稀释因子高的糯米香特征成分,结合双色谱柱保留指数、气味属性、一维和二维保留时间(1D Rt和 2D Rt)、高分辨质谱信息,成功将该糯米香特征成分鉴定为2-乙酰基-1-吡咯啉(2-acetyl-1-pyrroline,2AP),并通过香味物质添加实验对2AP的糯米香香韵贡献进行了验证.[结论]明确了烤烟烟叶中糯米香特征贡献组分为2AP,该物质在空气中阈值低至0.02×10-9,是典型的痕量高效香气化合物.研究结果为进一步开展糯米香烤烟精准评价和基于分子育种的烟叶糯米香特征的调控改良提供了物质基础.
Abstract
[Objective]The objective of this study was to accurately identify the key characteristic com-ponents responsible for the glutinous rice-like aroma in flue-cured tobacco.[Method]Flue-cured tobacco leaves exhibiting the glutinous rice-like aroma were screened and accurately identified using modern mo-lecular sensory science and technology.Gas chromatography olfactory detection/aroma extract dilution analysis(GC-O/AEDA)and multi-dimensional gas chromatography-high resolution mass spectrometry(GC×GC-QTOF/MS)were utilized for accurate identification.[Result]A trace potent odorant with a dis-tinct glutinous rice-like aroma,exhibiting high odor intensity,detection frequency,and dilution factor,was successfully located and screened from the flue-cured tobacco samples displaying the glutinous rice-like aroma.Through comparison of retention index on two different columns,odor properties,one-dimensional and two-dimensional retention times(1DRt and 2DRt),and high-resolution mass spectrometry information with an authentic standard,the unknown compound with a strong glutinous rice-like odor was unequivocally identified as 2-acetyl-1-pyrroline(2AP).An aroma components addition experiment was conducted to con-firm the flavor contribution of 2AP.[Conclusion]2AP was identified as the key odorant responsible for the glutinous rice-like aroma characteristic in flue-cured tobacco leaves.With an odor threshold as low as 0.02×10-9 in air,2AP was considered a typical trace but highly potent aroma-active compound.These find-ings provided a foundation for the accurate evaluation of flue-cured tobacco with glutinous rice-like aroma characteristics and for the regulation and enhancement of glutinous rice-like aroma in tobacco leaves through molecular breeding.
关键词
感官组学/糯米香香韵/痕量关键香气/气相色谱嗅觉检测技术(GC-O)/全二维气相色谱-四极杆飞行Key words
sensomics/glutinous rice-like aroma/trace potent odorant/gas chromatography-olfactometry/aroma extract dilution analysis(GC-O/AEDA)/comprehensive two-dimensional gas chromatography-quadrupole time-of-flight mass spectrometry(GC×GC-QTOF/MS)引用本文复制引用
基金项目
中国农业科学院科技创新工程项目(ASTIP-TRIC07)
江西中烟工业有限责任公司科技项目(赣烟工科2021-11)
中国烟草总公司山东省公司科技项目(201911)
中国烟草总公司贵州省公司科技项目(2021520000240044)
出版年
2024
NETL
NSTL
万方数据