Effects of FSLE on oxidative stress and inflammatory factors in APAP-induced liver injury mice
[Objective]This study aimed to investigate the impact of the ethanolic extract of Forsythia leaves(FSLE)on acetaminophen(APAP)-induced liver injury and its underlying mechanism.[Method]Forty-eight Kunming mice were randomly assigned to six groups:the blank control group(NC),APAP liver injury model group(LD),FSLE control group(FSLE),and high,medium,and low doses of FSLE treatment groups(HFSLE+LD,MFSLE+LD,and LFSLE+LD),with eight mice in each group.The liver injury model was established,and drug administration took place.The levels of alanine aminotransfer-ase(ALT),aspartate aminotransferase(AST),superoxide dismutase(SOD)activity,glutathione(GSH),malondialdehyde(MDA),and hydrogen peroxide(H2O2)were assessed in each group of mice.Liver histo-pathological changes were observed through hematoxylin-eosin staining(HE).TRAF2,NF-κB p65 mRNA,and protein expression levels were measured,along with TNF-α,IL-1β,and IL-6 levels.[Result]FSLE reduced ALT and AST activity,MDA,and H2O2 content,while increasing SOD activity and GSH content.It also ameliorated liver histopathology,decreased TRAF2 and NF-κB p65 mRNA and protein expression,and lowered TNF-α,IL-1β,and IL-6 levels in mice with liver injury.[Conclusion]In conclusion,FSLE demonstrated hepatoprotective effects against APAP-induced liver injury by mitigating oxidative stress,modulating the TNF/NF-κB p65 signaling pathway,and suppressing the inflammatory re-sponse.
ethanolic extract of Forsythia suspense leavesliver injurymiceoxidative stressinflamma-tory factors
万方数据
维普
