Histomorphological comparison of reproductive organs between adult and prepubertal mice after superovulation
[Objective]To study the histomorphological changes of the reproductive organs after super-ovulation in prepubertal mouse models,and to provide a theoretical reference for the implementation of su-perovulation in domestic animals.[Method]Twelve prepubertal mice and twelve adult mice of the same body weight were randomly divided into control group and experimental groups,with 6 mice in each group.After hormonal superovulation treatment,gross anatomy,tissue sections and transmission electron micros-copy were combined to analyze the morphological differences of the reproductive organs between prepuber-tal and adult mice.[Result]The adult superovulated group had a significantly lower number of corpus lutea than the prepubertal superovulated group(P<0.05),but a significantly higher area and diameter of the cor-pus luteum than the prepubertal superovulated group(P<0.01).Ultrastructure showed abundant lipid drop-lets in granular luteal cells and membranous luteal cells,and the granular luteal cells in the adult superovu-lated group were more closely arranged than those in juvenile superovulated group.Compared with that of the adult control group,the uterine cavity epithelium in the adult superovulated group was significantly rolled up.In addition,the uterine horn width,uterine body width,uterine horn area and uterine horn diameter of the adult superovulated group were significantly lower than those of the adult control group(P<0.01 or P<0.05).Uterine horn diameter,circular muscle layer thickness and longitudinal muscle layer thickness of the prepubertal mice in the superovulated group were significantly higher than those of the prepubertal mice in the control group(P<0.01 or P<0.05).[Conclusion]Compared to adult mice,prepubertal mice ovulate more frequently,but they produce a smaller area of mature follicles,which may affect the quality of the eggs.The uteri of adult and prepubertal mice respond differently to hormones that induce superovulation.
万方数据
维普
