In vivo visualization of renal serotonin dynamics using magnetic resonance imaging for early non-invasive diagnosis of drug-induced acute kidney injury
[Objective]Magnetic resonance imaging(MRI)was employed to monitor the real-time al-terations in renal serotonin receptors in vivo,presenting a non-invasive imaging technique for the prompt identification of drug-induced acute kidney injury.[Method]A magnetic resonance contrast agent(HT-Gd)engineered with serotonin receptor-targeting capabilities was developed based on the serotonin group.By analyzing the T1-weighted magnetic resonance imaging(T1WI)signals of serotonin receptors in mouse kidneys,an early MRI detection approach was investigated for the identification of drug-induced kidney in-jury.[Result]In vitro endocytosis experiments revealed that the uptake of HT-Gd by renal tubular epithe-lial cells(HK-2)was 36.8 pg/cell following a 12-hour cisplatin pretreatment,contrasting with a mere 14.7 pg/cell uptake in cells devoid of cisplatin treatment.In vivo magnetic resonance imaging studies dem-onstrated that HT-Gd heightened the renal T1WI signal-to-noise ratio to 40.5%in mice subjected to cis-platin treatment for 24 hours,approximately double that of mice not treated with cisplatin(SNR=20.2%).[Conclusion]The visualization of serotonin receptors in the kidney using HT-Gd has enabled the early diagnosis of cisplatin-induced acute kidney injury,offering a novel reference technique for the early di-agnosis and treatment exploration of drug-induced acute kidney injury.
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