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促卵泡激素受体在双峰驼附睾的定位及分布特点

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[目的]研究促卵泡激素受体(follicle-stimulating hormone receptor,FSHR)对双峰驼附睾发育及生殖机能调节的影响.[方法]应用H.E染色、糖原PAS和免疫组织化学SP法结合Image J统计方法,分析促卵泡激素受体在双峰驼附睾的组织学特性.[结果]H.E染色结果显示,双峰驼附睾各部分管腔为柱状纤毛上皮,成年双峰驼附睾管壁上皮细胞主要由主细胞、基细胞等组成.附睾管头部和附睾管尾部PAS阳性反应强于附睾管体部;免疫组化及免疫荧光结果表明,FSHR在双峰驼附睾头体尾均有表达,FSHR在成年双峰驼附睾管中头部和尾部的表达强度显著高于体部;在附睾头以纤毛上皮细胞游离缘及间质血管内皮细胞为主,FSHR在附睾体及附睾尾以基细胞表达为主.[结论]FSHR在双峰驼附睾中与精子的成熟以及基细胞介导细胞的吞噬及保护机制有关.
Location and distribution of follicle-stimulating hormone receptor in the epididymis of bactrian camel
[Objective]The aim of the study was to investigate the effects of follicle-stimulating hor-mone receptor(FSHR)on the regulation of epididymal development and reproductive function in bactrian camel.[Method]The histological characteristics of FSH receptor in the epididymis of bactrian camel were analyzed by using the H.E staining,glycogen PAS and immunohistochemical SP methods combined with Image J statistical analysis.[Result]The H.E staining showed that the lumen of each part of the epididy-mis of bactrian camel was columnar ciliated epithelium,and the epithelial cells of the epididymal wall of adult bactrian camel were mainly composed of main cells and basal cells.The PAS positive reactions both in the head and tail of the epididymal duct were stronger than those in the body of the epididymal duct.The results of immunohistochemistry and immunofluorescence showed that FSHR was expressed in the head,body and tail of camel epididymis.The expression levels of FSHR in the epididymal duct of adult camel were significantly higher in the head and tail than in the body.The free margin of ciliated epithelial cells and interstitial vascular endothelial cells were dominant in the caput epididymis,and FSHR was mainly ex-pressed in basal cells in the epididymal body and cauda epididymis.[Conclusion]FSHR are associated with sperm maturation and involved in the mechanism of basecell-mediated cell phagocytosis and protection in the epididymis of bactrian camel.

bactrian camelepididymisfollicle stimulating hormone receptorimmunohistochemistry

张芳、袁莉刚、马晓杰、王华、杨大鹏

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甘肃农业大学动物医学院,甘肃 兰州 730070

双峰驼 附睾 促卵泡激素受体 免疫组织化学

2024

甘肃农业大学学报
甘肃农业大学

甘肃农业大学学报

CSTPCD北大核心
影响因子:0.612
ISSN:1003-4315
年,卷(期):2024.59(6)