摘要
[目的]研究水扬酸(SA)引发处理对辣椒植株盐胁迫的缓解效应及机制.[方法]以朝天椒茂蔬360为原料,采用水杨酸溶液为种子萌发处理,通过100 mmol/L氯化钠溶液模拟盐胁迫,以不同浓度的(0、0.05、0.1、0.2、0.3、0.4、0.5和0.6 mmol/L)水杨酸溶液对朝天椒种子进行引发处理,分析其对朝天椒的种子萌发、植株生长、叶绿素含量、抗氧化酶活性、渗透调节物质含量和钾、钠离子积累的影响以及提高4～6叶期植株耐盐性的机制.[结果]0.1 mmo/L水杨酸溶液种子引发处理后能够显著提高盐胁迫下种子的发芽率和发芽势,同时缓解了4～6叶期植株在盐胁迫下的伤害,表现为植株矮壮且根系发达;相比未引发处理,叶片叶绿素含量显著升高,POD酶活性和GR酶活性增强,丙二醛含量显著下降,可溶性蛋白质和可溶性糖类物质含量增高,叶片钾、钠离子含量同步增加.[结论]0.1 mmol/L SA溶液种子引发处理可用于提高盐胁迫下的朝天椒种子萌发和缓解4～6叶期植株受盐胁迫的伤害,其作用机制是促进了光合作用,提高了活性氧的清除能力,促进了叶片钾离子积累.
Abstract
[Objective]Study on the alleviating effect and mechanism of salt stress in pepper plant in-duced by salicylic acid treatment.[Method]Taking Chaotian Pepper Maoshu 360 as material,using sali-cylic acid solution as seed initiation treatment.The seeds were treated with salicylic acid solution at 0,0.05,0.1,0.2,0.3,0.4,0.5 and 0.6 mmol/L through 100 mmol/L sodium chloride solution to simulate salt stress.The effects on seed germination,plant growth,chlorophyll content,antioxidant enzyme activity,os-motic regulators content and accumulation of potassium and sodium ions of pepper were analyzed,as well as the mechanism of improvement in plant salt tolerance at the 4～6 leaf stage.[Result]0.1 mmol/L salicylic acid solution seed initiation treatment could significantly improve the germination rate and germination po-tential of seeds under salt stress,and alleviate the damage of plants at 4～6 leaf stage under salt stress,show-ing that the plants were short and strong with developed roots.Compared with no treatment,chlorophyll content was significantly increased,POD enzyme activities and GR enzyme activities were enhanced,malo-ndialdehyde content was significantly decreased,soluble protein and soluble carbohydrate contents were in-creased,and potassium and sodium contents in leaves were simultaneously increased.[Conclusion]Seed treatment with 0.1 mmol/L SA solution can be used to improve seed germination and alleviate salt stress damage to plants at 4～6 leaf stages.The mechanism of action is to promote photosynthesis,improve the scavenging ability of reactive oxygen species,and promote the accumulation of potassium ions in leaves.
维普
万方数据