Salicylic acid treatment Chaotian pepper seeds and seedlings for salt stress relief effect
[Objective]Study on the alleviating effect and mechanism of salt stress in pepper plant in-duced by salicylic acid treatment.[Method]Taking Chaotian Pepper Maoshu 360 as material,using sali-cylic acid solution as seed initiation treatment.The seeds were treated with salicylic acid solution at 0,0.05,0.1,0.2,0.3,0.4,0.5 and 0.6 mmol/L through 100 mmol/L sodium chloride solution to simulate salt stress.The effects on seed germination,plant growth,chlorophyll content,antioxidant enzyme activity,os-motic regulators content and accumulation of potassium and sodium ions of pepper were analyzed,as well as the mechanism of improvement in plant salt tolerance at the 4~6 leaf stage.[Result]0.1 mmol/L salicylic acid solution seed initiation treatment could significantly improve the germination rate and germination po-tential of seeds under salt stress,and alleviate the damage of plants at 4~6 leaf stage under salt stress,show-ing that the plants were short and strong with developed roots.Compared with no treatment,chlorophyll content was significantly increased,POD enzyme activities and GR enzyme activities were enhanced,malo-ndialdehyde content was significantly decreased,soluble protein and soluble carbohydrate contents were in-creased,and potassium and sodium contents in leaves were simultaneously increased.[Conclusion]Seed treatment with 0.1 mmol/L SA solution can be used to improve seed germination and alleviate salt stress damage to plants at 4~6 leaf stages.The mechanism of action is to promote photosynthesis,improve the scavenging ability of reactive oxygen species,and promote the accumulation of potassium ions in leaves.