Physiological characteristics and metabolomics analysis of autotriploid Ulmus pumila
[Objective]The objective of this study was to investigate the genetic characteristics of physi-ological traits and metabolites in autotriploid Ulmus pumila and its parental species,laying a theoretical foundation for screening new germplasms of Ulmus pumila with enhanced adaptability and resistance.[Method]In this study,Autotriploid U.pumila and its parental species were used as the materials for as-sessing physiological parameters and conducting metabolite analysis using non-targeted liquid chromatogra-phy mass spectrometry.[Result]The results indicated that the antioxidant enzyme activity and photosyn-thetic rate of autotriploid Ulmus pumila were significantly higher than those of the maternal diploid Ulmus pumila and lower than those of the paternal tetraploid Ulmus pumila,with minor deviations from the paren-tal traits.Additionally,the malondialdehyde content was notably lower than that of the maternal diploid Ul-mus pumila and higher than that of the paternal tetraploid U.pumila,with minor differences from the paren-tal characteristics.A total of 1 055 substance signals were detected from Autotriploid Ulmus pumila and its parental samples,leading to the identification of 90 differential metabolites.These metabolites included vari-ous compounds such as 7,8-dihydroneopterin,Lychnose,L-glutamic acid,Quercetin,as well as sugars,amino acids,and flavonoids.Some of these compounds were found to be down-regulated in relative content compared to the parental species,while others were up-regulated.Furthermore,metabolites like Catechin,(S)-Pinocembrin,Hesperidin 7-O-glucoside,Sinapoyl malate,along with terpenoids,phenols,flavonoids,and phenylpropanoids,were found to be down-regulated in relative content compared to the parental spe-cies.Conversely,Pantothenic acid and Raffinose exhibited up-regulation compared to the parental species.The differential metabolites were predominantly enriched in the Flavonoid biosynthesis and Flavone and fla-vonol biosynthesis metabolic pathways.[Conclusion]Autotriploid Ulmus pumila exhibited significant dif-ferences in its metabolite profiles compared to its parental species.These differences in quantity and content may be closely associated with its antioxidant enzyme activity,photosynthetic rate,and resistance to abiotic stresses.
万方数据
维普
