首页|植物乳杆菌TY-Y10的特性研究

植物乳杆菌TY-Y10的特性研究

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[目的]评价植物乳杆菌TY-Y10(TY-Y10)的生长曲线、产酸能力、耐酸耐胆盐性能,研究其活菌及热灭活状态下的抗氧化能力.[方法]试验采用分光光度法测定菌株生长曲线,pH值变化曲线评估其产酸能力,平板计数法测定该菌株在酸性及高胆盐环境的活菌数及存活率.DPPH自由基清除率、羟自由基清除率和还原能力评价TY-Y10在活菌和不同温度下灭活菌的体外抗氧化能力.[结果]TY-Y10在MRS培养基中生长4 h后进入对数期,14 h后达到稳定期;pH在12 h后降至4.0以下;菌株在pH=2.5、3.0、3.5、4.0和4.5的环境下培养3 h,其存活率分别为69.62%、76.44%、102.68%、236.00%、570.80%.在胆盐浓度为0.1%、0.2%和0.3%的MRS培养基中培养8 h后,其存活率分别为109.94%、101.95%、100.23%.TY-Y10活菌菌体、80℃热灭活菌体、100℃热灭活菌体的DPPH清除率为38.38%、41.37%、32.41%,羟自由基清除率为80.55%、90.53%、76.80%,还原能力为169、231、93 μmol/L 的L-半胱氨酸盐酸盐当量;TY-Y10活菌上清液、80℃热灭活上清液、100℃热灭活上清液的DPPH清除率为29.97%、15.85%、14.22%,羟自由基清除率为32.64%、48.00%、67.83%,还原能力为72、128、66 μmol/L 的L-半胱氨酸盐酸盐当量.[结论]TY-Y10繁殖能力强,具有较强的产酸、耐酸、耐胆盐能力,活菌和热灭活TY-Y10具有显著的体外抗氧化活性,且80℃热灭活菌体抗氧化能力最强.
Study on the characteristics of Lactobacillus plantarum TY-Y10
[Objective]The objective of the study was to assess the growth curve,acid production capac-ity,acid tolerance,and bile salt tolerance of Lactobacillus plantarum TY-Y10(TY-Y10)and to investigate its viability and antioxidant properties under heat inactivation.[Method]The growth curve of the strain was determined using spectrophotometry,acid production capacity was evaluated through pH change curves,and the survival rate of viable bacteria in acidic and high-bile salt environments was determined through plate counting.The antioxidant capacity of TY-Y10 was assessed using DPPH and hydroxyl free radical scaveng-ing rates as well as reducing ability under live and heat-inactivated conditions at various temperatures.[Result]Our results showed that TY-Y10 entered the logarithmic phase after 4 hours of growth in MRS medium and reached a stable phase after 14 hours.The pH dropped below 4.0 after 12 hours.The survival rates of the strain were 69.62%,76.44%,102.68%,236.00%and 570.80%when cultured in environ-ments with pH values of 2.5,3.0,3.5,4.0 and 4.5 for 3 hours.In the presence of bile salt concentrations of 0.1%,0.2%,and 0.3%in MRS medium,the survival rates were 109.94%,101.95%,and 100.23%after 8 hours,respectively.The DPPH clearance rates for TY-Y10 live bacteria,80℃heat-inactivated bacteria,and 100℃heat-inactivated bacteria were 38.38%,41.37%and 32.41%,respectively.The hydroxyl free radical clearance rates were 80.55%,90.53%and 76.80%,and the reducing ability was 169,231 and 93 μmol/L of L-cysteine hydrochloride equivalent.Moreover,the DPPH clearance rates for TY-Y10 live bac-teria supernatant,80℃heat-inactivated supernatant,and 100℃heat-inactivated supernatant were 29.97%,15.85%and 14.22%,respectively.The hydroxyl radical clearance rates were 32.64%,48.00%,and 67.83%,and the reducing ability was 72,128 and 66 μmol/L of L-cysteine hydrochloride equivalent.[Conclusion]In conclusion,TY-Y10 demonstrated robust reproductive ability,strong acid production,acid resistance,and bile salt resistance.Both live and heat-inactivated TY-Y10 exhibited significant in vitro anti-oxidant activity,with the 80℃ heat-inactivated bacteria showing the highest antioxidant activity.

Lactobacillus plantarumantioxidationgrowth abilityheated-killed

石韶琦、王然、张凤、王剑、蒋源渊、张琪、王静、何晶晶、文鹏程、张炎

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甘肃农业大学食品科学与工程学院,甘肃 兰州 730070

中国农业大学营养与健康系,北京 100190

重庆市天友乳业股份有限公司,重庆 401120

植物乳杆菌 抗氧化 生长能力 热灭活

2024

甘肃农业大学学报
甘肃农业大学

甘肃农业大学学报

CSTPCD北大核心
影响因子:0.612
ISSN:1003-4315
年,卷(期):2024.59(6)