Screen of Amylase-producing Bacterial Strains of Tabacco and Enzyme Production Conditions Optimize
Based on 224 of amylase-producing bacterial strains isolated from tobacco leaves and their screening for the production of amylase in our previous work,this study aimed to screen amylase-producing bacterial strains which could obviously improve tobacco interior quality by the assessment of sense organ wih smoking,including amylase A-7,A-8,A-9. Different concentrations of the enzyme solution to improve the quality of tobacco leaves shows that 0.29 U/mL of the enzyme solution A-7, 80.67 U/mL of A-8,213.33 U/mL of A-9 on tobacco smoking indicators have improved. The conditon for enzyme production from strain amylase-producing bacterial A-8 is optimized. The result shows that A-8 at pH 6.0,37 ℃conditions,shaking culture 36 hours biomass maximum,60 hours highest activity,the total enzyme activity could be improved by 2.7 times under the optimum conditions.
国家科技期刊平台
NSTL
万方数据
维普
