Identification of the BBX family in B.rapa and Stress Response Analysis of BrBBX46 and BrBBX53 Genes
BBX is an important family of zinc finger protein transcription factors,containing one to two highly conserved BBX domains located at the N terminus of the protein sequence.It plays an important role in regulating plant growth and development and responding to environmental stress.In this study,59 BrBBX family members were identified in B.rapa by bioinformatics analysis,and their chromosome location,gene structure,protein conserved domain,and promoters of BrBBX46 and BrBBX53 genes were analyzed.The expression levels of BrBBX46 and BrBBX53 were determined by qRT-PCR under simulated drought and salt stress.The result showed that 59 BrBBXs were unevenly distributed on 10 chromosomes of the B.rapa genome.Based on the analysis of phylogenetic evolution,gene structure and protein conserved domains,59 BrBBXs were divided into five subfamilies.The gene structure,protein domain and conserved motifs of each subfamily were relatively conserved,and members of the same subfamily had similar gene structure and conserved domains.The results of promoter analysis showed that the promoters of BrBBX46 and BrBBX53 contained a large number of stress-related response elements,especially drought stress response elements,and there were 14 drought stress response elements in BrBBX46 and BrBBX53,respectively.The expression levels of BrBBX46 and BrBBX53 were significantly increased under the treatment of 40%PEG and 120 mM NaCl,indicating that BrBBX46 and BrBBX53 can respond to the induction of drought and salt stress and may play important functions under drought and salt stress.These results provide good gene resources for further understanding of BrBBX family and using BrBBX family members to improve the drought and salt tolerant new varieties of B.rapa.
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