As the fifth member of the protein family Anostatin,the ANO5 gene mainly plays a role in sheep muscle regeneration,myocyte fusion,and muscle cell membrane repair.To explore the function of ANO5 gene in sheep,bioinformatics software was used in this study to analyze the structure and function of the ANO5 gene and its encoding product.The findings indicatesthat the ANO5 gene encodes 1116 amino acid residues and has an open reading frame with a maximal length of 3 351 bp.The protein that is encoded by this gene is C5897H9014N1546O1668S42 in molecular formula,129 602.32 Da in molecular mass,7.24 in theoretical isoelectric point,30 hours in estimated half-life,49.71 in instability index,and 79.43 in aliphatic amino acid index.The sheep ANO5 protein,which contains eight transmembrane domains,is an unstable hydrophilic transmembrane proteinand is most likely to be found in the plasma membrane(56.5%).It also lacks signal peptides.Random curling accounted for 68.43%of the secondary structurewhile winding folding,and random curling constituted the majority of the tertiary structure.
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