Study on Tissue Culture and Rapid Propagation Techniques of Apple Dwarf Rootstock M9T337
Dwarfing cultivation is the developmental trend of modern apple production.Promotion and application of dwarfing rootstock is the main way to realize apple dwarfing cultivation,standardized management for apple production.To improve and optimize the tissue culture and regeneration system of apple dwarf rootstocks,in this study,apple dwarfing rootstock M9T337 was used to screen the best disinfection plan for explants using 75%ethanol and 1 g/kg mercuric chloride,respectively.M9T337 stem-segment with single bud was used to study the effects of different culture-medium combinations on the pollution rate,multiplication coefficient,plant height,growth,rooting number,and rooting rate of tissue culture seedlings.The results showed that 75%ethanol for 30 s + 1 g/kg mercuric chloride for 9 min had the best disinfection effect.The optimal formulations of primary medium,secondary medium and rooting medium were MS+6-BA 0.8 mg/L+NAA 0.4 mg/L,MS+BA 1.0 mg/L+NAA 0.5 mg/L and 1/3MS+IBA 0.1 mg/L,respectively.In this experiment,the efficient in vitro rapid propagation technology system of apple dwarf rootstock M9T337 was established,and the best propagation and rooting medium suitable for M9T337 were selected as well.
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