胶体金免疫层析法在韭菜农药残留检测中的应用研究
Study on the Application of Colloidal Gold Immunochromatography in the Detection of Pesticide Residues in Chinese Chives
张继梅 1冯文霞 1白新明 1李亚莉2
作者信息
- 1. 平凉市农产品质量安全与检验检测中心,甘肃 平凉 744000
- 2. 甘肃省农业科学院蔬菜研究所,甘肃 兰州 730070
- 折叠
摘要
为验证胶体金免疫层析技术在韭菜农残检测中的可靠性和适用性,选用 3 种不同的农残胶体金免疫层析快速检测产品(胶体金农残快检试剂)检测韭菜中腐霉利、吡虫啉、啶虫脒、多菌灵、克百威、三唑磷等 6种农药残留,并将其与色谱质谱串联法进行比较,对其灵敏度、假阳性率及假阴性率进行验证评价.结果表明,在 0.5 LOD的农药添加水平下,3种胶体金农残快检试剂对实验室空白样品的检测结果均为阴性;但当添加水平达到LOD或MLR时,其检测结果均为阳性,证明胶体金农残快检试剂灵敏度较高,且符合国家标准食品中农药最大残留限量要求;实验室加标试验和实际样品比对试验结果显示,胶体金农残快检技术与色谱质谱联用法的检测结果均无显著性差异(χ2<3.84),其假阴性率和假阳性率均为 0.故胶体金免疫层析法可在韭菜的农残快速检测中应用.
Abstract
To validate the reliability and applicability of colloidal gold immunochromatographic technology in the detection of pesticide residues in Chinese chives,3 different types of colloidal gold immunochromatographic rapid test products(colloidal gold pesticide rapid test reagents)were used to detect 6 types of pesticide residues,including fludioxonil,imidacloprid,thiamethoxam,carbendazim,cypermethrin,and triazophos,in Chinese chives.These results were compared with those obtained using chromatography-mass spectrometry tandem method,assessing their sensitivity,false positive rate,and false negative rate.The results showed that at 0.5 LOD pesticide addition level,all 3 colloidal gold pesticide rapid test reagents yielded negative results for laboratory controls.However,when the addition levels reached LOD or MLR,the results were positive,demonstrating high sensitivity of the colloidal gold pesticide rapid test reagents,which comply with the national standards for maximum residue limits in food.Laboratory spike tests and actual sample comparison tests showed no significant differences(χ2<3.84)between the colloidal gold rapid detection technology and the chromatography-mass spectrometry method,with both false negative and false positive rates being 0.Therefore,colloidal gold immunochromatographic method can be applied in the rapid detection of pesticide residues in Chinese chives.
关键词
韭菜/农药残留/胶体金免疫层析法/快速检测/色谱质谱联用法Key words
Chinese chive/Pesticide residue/Colloidal gold immunochromatography/Rapid test/Chromatography-mass spec-trometry tandem method引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据