NaCl胁迫对春小麦苗期叶绿素及保护性酶活性的影响
Effects of NaCl Stress on Chlorophyll Contents and Protective Enzyme Activities of Spring Wheat at Seedling Stage
王婵 1侯格平 1朱妍钰 2马君君 2王万鹏 1李元昊 2吴海燕 1惠永芳 2刘鹏伟 2张文宇 2马小乐2
作者信息
- 1. 张掖市农业科学研究院,甘肃 张掖 734000
- 2. 甘肃农业大学农学院,甘肃 兰州 730070
- 折叠
摘要
为了探究春小麦苗期的耐盐状况及其生理特性,为耐盐品种的筛选提供理论依据,以 6 个春小麦品种(系)为材料,研究其在不同浓度NaCl胁迫下的生理特性及耐盐状况.结果表明,NaCl浓度为 0.9、1.2 mol/L的高盐胁迫后第 20、30 天,所有供试材料均枯萎死亡,说明春小麦对NaCl胁迫的耐受浓度不能高于 0.9 mol/L.随着NaCl胁迫强度增强和时间延长,叶绿素含量逐渐下降,但在NaCl浓度为 0.3、0.6 mol/L的盐胁迫处理下各供试材料差异不显著,说明小麦苗期可耐受 0.6 mol/L NaCl胁迫.CAT活性均表现为先升后降的趋势,而MDA和Pro含量呈现上升趋势,说明NaCl胁迫可诱导小麦苗期保护性酶的活性.综合分析,甘春 28 号和品系H-5的耐盐性较强,可在盐碱地进一步试验种植.
Abstract
In order to study the salt tolerance and physiological characteristics of spring wheat at seedling stage,aiming to provide theoretical basis for the screening of salt-tolerant varieties,this experiment took 6 spring wheat varieties as materials to study their physiological characteristics and salt tolerance under different NaCl concentrations.The results showed that under high salt stress of 0.9 mol/L and 1.2 mol/L,all varieties withered and died after 20 days or 30 days of stress treatment,indicating that the tolerance concentration of spring wheat to NaCl stress could not be higher than 0.9 mol/L.With the increase of NaCl stress intensity and the extension of NaCl stress time,chlorophyll content decreased gradually,but the difference between 0.3 mol/L and 0.6 mol/L treatment was not significant,indicating that wheat could tolerate 0.6 mol/L NaCl stress at seedling stage.The activity of CAT increased first and then decreased,while the content of MDA and Pro increased,indicating that NaCl stress could induce the activity of protective enzymes in wheat seedling stage.By analyzing the difference of varieties tested,it was found that Ganchun 28 and strain H-5 had strong salt tolerance and could be planted in saline-alkali soil for further experiment.
关键词
春小麦/NaCl胁迫/耐盐性/保护酶活性Key words
Spring wheat/NaCl stress/Salt tolerance/Protective enzyme activity引用本文复制引用
基金项目
张掖市重大技术攻关揭榜挂帅制项目(ZY2022JBGS08)
甘肃省教育厅产业支撑计划项目(2021CYZC-12)
甘肃省农业科技支撑项目(KJZC-2023-2)
甘肃农业大学2024年SIETP项目(202401039)
甘肃农业大学2024年SIETP项目(202401038)
出版年
2024
国家科技期刊平台
NSTL
万方数据