Optimization of Lentinula edodes Spawn Medium Formula Based on D-optimal Method
In order to screen and optimize the culture medium formula of Lentinula edodes original and cultivated species,the D-optimal design method was used to optimize the original culture medium of Lentinula edodes with different ratios of wheat grain and sawdust as raw materials,and the cultivation medium of Lentinula edodes with different ratios of sawdust,corncob and bran as raw materials.The Lentinula edodes variety L808 was used as the test strain,and the mycelial germination period,mycelial growth rate and full bag period were used as the evaluation indexes,respectively.Through the measurement of the indexes,the regression model between the ratio matrix and the response value of the Lentinula edodes culture medium formula was established,so as to scientifically optimize the formula of the original and cultivated species of Lentinula edodes.The results showed that the optimal formula of Lentinula edodes original culture was:50%wheat grain+50%sawdust;the optimal formula of Lentinula edodes cultivation was:37.69%corncob+23.33%bran+38.98%sawdust.After inoculating the cultivation substrates of the above two formulas,the mycelium of Lentinula edodes grew vigorously,with a short germination period and a short full bag period,and the physicochemical properties were also superior,indicating that the optimized cultivation substrate formula is highly feasible.This design method is scientific and feasible in optimizing the ratio of cultivation materials.
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