外源物质对盐碱胁迫下垂丝海棠生长及生理特性的影响
Effects of Exogenous Substances on the Growth and Physiological Characteristics of Malus halliana under Salt-alkali Stress
达建东 1郭润霞 2高立杨 3李平1
作者信息
- 1. 武威市农业技术推广中心,甘肃 武威 733000
- 2. 武威市农业科学研究院,甘肃 武威 733000
- 3. 武威市农业农村局,甘肃 武威 733000
- 折叠
摘要
为探究褪黑素和2,4-表油菜素内酯在盐碱胁迫下对垂丝海棠生长及其生理特性的影响,以 9～10叶龄垂丝海棠实生苗为材料,设置 200 μmol/L褪黑素喷施、200 μmol/L褪黑素+0.2 mg/L 2,4-表油菜素内酯叠加喷施、0.2 mg/L 2,4-表油菜素内酯喷施 3 个处理,研究其对垂丝海棠盐碱胁迫下叶片叶绿素含量、相对电导率、含水量、抗氧化酶活性、游离脯氨酸含量等生理指标和光合荧光参数的影响.结果表明,外源喷施褪黑素和 2,4-表油菜素内酯可显著提高盐碱胁迫下垂丝海棠叶片的相对导电率、脯氨酸含量、抗氧化酶活性,降低叶片相对含水量、叶绿素含量,从而缓解盐碱胁迫对垂丝海棠生长的抑制.本试验中,缓解效果由高到低依次为褪黑素、2,4-表油菜素内酯、二者叠加使用.
Abstract
In order to explore effects of melatonin and 2,4-epibrassinolide to alleviate the damage of combined salt-alkali stress on Malus halliana,in this experiment,the seedlings of Malus halliana with 9 to 10 leaf ages were used as the test material.Three treatments were set,i.e.,200 μmol/L melatonin spray,200 μmol/L melatonin+0.2 mg/L 2,4-table brassinolide overlay spray and 0.2 mg/L 2,4-table brassinolide spray,to study the effects on chlorophyll content,relative conductivity,water content,antioxidant enzyme activity,free proline content and photosynthetic fluorescence parameters of leaves under salt-alkali stress.The results showed that exogenous application of melatonin and 2,4-epbrassinolide could significantly improve the relative conductivity,proline content and antioxidant enzyme activity of leaves under salt-alkali stress,and reduce the relative water content and chlorophyll content of leaves,so as to alleviate the inhibition of salt-alkali stress on the growth of Malus linus.In this experiment,the alleviating effect from high to low was melatonin,2,4-epbrassinolide,and the combination of the two.
关键词
垂丝海棠/盐碱胁迫/褪黑素/2,4-表油菜素内酯Key words
Malus halliana/Salt-alkali stress/Melatonin/2,4-epibrassinolide引用本文复制引用
基金项目
武威市自然科学基金(WW23A03RPZ006)
出版年
2024
国家科技期刊平台
NSTL
万方数据