Study on Embryonic Callus Induction and Plant Regeneration of Heuchera micrantha
In order to establish tissue culture and rapid propagation system of Heuchera micrantha,using the young terminal buds of Heuchera micrantha as explants,this study investigated the effects of different media on the induction,proliferation,and regeneration of embryogenic callus tissues.The results showed that the medium MS+2.4-D 3.0 mg/L+6-BA 0.3 mg/L had a high callus induction rate of 93.0%.The callus formed was large,loosely structured,and grainy but immature,with some clusters appearing water-soaked.For the proliferation of embryogenic callus tissues,the medium MS+2.4-D 2.0 mg/L+6-BA 0.2 mg/L resulted in well-developed callus tissues that were loosely structured with distinct granular nodules and less water content,presenting a light yellow-green color.The differentiation medium MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+IBA 0.2 mg/L achieved a differentiation rate of 91.8%.For plants with poorly developed young roots,rooting culture on 1/2 MS+IBA 0.1 mg/L+IAA 0.1 mg/L resulted in a rooting rate of 100%,with an average of 5.5 roots per plant,root lengths of 2 to 3 cm,and plant height of 4.1 cm.
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