Isolation,Identification and Antagonism of Biocontrol Trichoderma spp.from Rhizosphere Soil of Angelica sinensis
Soil samples were collected from the rhizosphere of authentic medicinal herb Angelica sinensis in Gansu Province,and Trichoderma spp.strains were isolated using the dilution coating method.The antagonistic effects were determined using the plate confrontation method,and the classification of Trichoderma spp.strains were identified through morphological characteristics and ITS sequence alignment,aiming to screen strains with biocontrol potential and clarify their antagonistic effects.The results showed that 21 Trichoderma spp.strains were obtained by isolated from the rhizosphere soil of Angelica Sinensis in Gansu Province,1 strain with the best inhibitory effect on the target pathogens was screened and identified as Trichoderma longibrachiatum strain TD-5.TD-5 had the strongest antagonistic effect on Fusarium solani with an inhibitory rate at 83.31%,antagonistic effect against Phyllosticta capitalensis was the worst(inhibitory rate was 24.55%),the antagonism effects against Fusarium tricinctum and Colletotrichum gloesosporioides were between them.The volatile metabolites of TD-5 were most sensitive to Fusarium solani(inhibitory rate was 79.44%),the sensitivity to Colletotrichum gloesosporioides was the lowest(inhibitory rate was 64.99%);Non-volatile metabolites from strain TD-5 were most sensitive to Fusarium tricinctum(inhibitory rate was 70.72%),the sensitivity to Fusarium solani was the lowest(inhibitory rate was 31.12%).
Angelicae sinensisTrichoderma spp.Biocontrol bacteriaIsolation and identificationAntagonism