Isolation and Identification of the Pathogen Causing Black Rot of Cabbage in Gansu Province
To clarify the pathogen causing cabbage black rot in Zhangye City,Gansu Province,the cabbage leaves with typical disease symptoms were collected for pathogen isolation,purification by using plate scribing method.The pathogenicity was determined,and pathogen also was identified by morphological characteristics and molecular biology methods.The results showed that 6 isolates were obtained,among which GL3-2,Gram-negative,was pathogenic bacteria.Its colony was round,light yellow at first,and then honey yellow gradually.The colony edges were neat,smooth,and glossy;Most of the bacteria were short rods with the size of(0.5 to 1.0)μm×(1.0 to 3.0)μm.No spores,solitary or chained,extremely solitary flagella.GL3-2 was identified as Xanthomonas campestris pv.campestris based on culture traits,morphological characteristics,16S rDNA and specific genes sequence analysis.
Black rot of cabbagePathogenIsolationIdentification
国家科技期刊平台
NSTL
万方数据
