Effects of PEG-6000 Simulated Drought Stress on Physiological Characteristics of Bupleurum smithii var.Parvifolium Seedlings
By exploring the response of Bupleurum smithii var.parvifolium to drought stress,this study provides scientific evidence for comprehensively evaluating its drought tolerance,further offering technical guidance for field cultivation.Using Bupleurum smithii var.parvifolium seedlings as experimental materials,the drought stress was simulated with PEG-6000,and the effects of drought on leaf antioxidant enzyme activity,cell membrane permeability,and osmotic regulatory substances were studied.The results showed that with increasing PEG-6000 concentrations(0 to 25%),the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),as well as contents of malondialdehyde(MDA),free proline(Pro),and soluble sugar in the leaves all exhibited similar trends,first increasing and then decreasing,although the peak values varied slightly.The MDA content continued to increase as the PEG-6000 stress reached its highest concentration(25%).Overall,the study concluded that Bupleurum smithii var.parvifolium possesses certain drought tolerance and can maintain osmotic stress resistance and reactive oxygen species scavenging abilities under moderate drought stress,thereby maintaining membrane stability.
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