Systematic Breeding and SSR Fingerprint of the New Foxtail Millet Variety Longgu 23
Millet,as one of the characteristic crops in northern China,mostly utilizes conventional breeding methods combined with a few molecular-assisted selection techniques.Constructing a fingerprint map is a key component of molecular breeding,which is significant for variety identification and authenticity verification.Longgu 23,a new foxtail millet variety with high quality,and high yield,were bred through sexual hybridization between Yugu 1 and Longgu 7.In 2020,the average yield of Longgu 23 was 5 446.0 kg/ha in seven pilot areas of the joint national identification experiment for the early maturing area of northwest spring millet with a yield increase rate of 57.14%.During the millet production experiments in Gansu Province in 2020,it demonstrated yield increases at all 8 points with an average yield of 4 537.5 kg/ha,which was an increase of 2.08%to 36.84%compared to the control variety Yugu 18.The contents of crude protein(dry basis),ether extract(dry basis),crude starch(dry basis),lysine(dry basis),and amylopectin(accounting for starch)were 125.7 g/kg,38.2 g/kg,794.5 g/kg,1.8 g/kg and 784 g/kg,respectively.It is resistant to grain rust and smut,moderately resistant to grain blast,white hair disease,and corn borer,and is suitable for planting in northwestern spring sowing areas such as central and eastern Gansu,Datong in Shanxi,Yulin in Shaanxi,Guyuan in Ningxia,and Hohhot in Inner Mongolia.In addition,74 pairs of polymorphic SSR markers in the linkage map constructed by the F2 and RIL populations from Yugu 1 × Longgu 7 was used to map the 9 chromosomes of Longgu 23.Among them,49 pairs of SSR genotypes came from Yugu 1 and 25 pairs of SSR genotypes were same to Longgu 7,indicating that Longgu 23 carries more components of the Yugu 1 genome.This fingerprint provides a molecular basis for the authenticity identification of Longgu 23.