Study on the Key Factors Affecting Rooting in Sugar-free Tissue Culture of Peach Rootstock GF677
Addressing the issue of peach heavy stubble is of great significance for the renewal and transformation of old peach orchards.Peach rootstocks are the basis for peach tree grafting.To provide a scientific basis for the rapid propagation of replantation-resistant peach rootstocks using sugar-free tissue culture technology,experiments were conducted on effects of adding different concentrations of improved QL nutrient solution,various concentrations of IBA,and different substrate formulations on the rooting of the superior GF677 rootstock.A comparative experiment between traditional tissue culture rooting and sugar-free tissue culture rooting was also carried out.Results showed that using 50.00％improved QL nutrient solution in sugar-free tissue culture resulted in the best rooting effect,with a rooting rate of 96.67％,an average of 3.6 in root number,and a root length of 3.45 cm.When the IBA concentration in the nutrient solution was 1.25 mg/L,the rooting effect was optimal,with a rooting rate of 96.67％,4.13 in root number,and a root length of 3.60 cm.The best rooting effect was observed when pure vermiculite was used as the substrate,with a rooting rate of 93.33％,3.80 in root number,and a root length of 3.50 cm.Additionally,it was found that the rooting rate in sugar-free tissue culture was generally higher than that in the traditional tissue culture,with the highest rooting rate occurring at 1.25 mg/L IBA,which showed a significant difference compared to traditional tissue culture.The transplant survival rate of GF677 peach rootstock in sugar-free tissue culture was 92.6％,which was an increase of 4.6 percentage points over the traditional tissue culture.Therefore,the rational use of key factors for sugar-free tissue culture rooting(nutrient solution,IBA concentration,and substrate formulation)can significantly improve the rooting rate and survival rate of GF677 peach rootstock,and should be widely promoted and applied.
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