摘要
目的:在单因素试验的基础上,采用响应面试验优化超声辅助酶法提取鸡骨草多糖的最佳工艺,并通过体外实验研究其抗炎活性.方法:单因素试验考查加酶量、液料比、酶解pH、酶解-超声功率、酶解-超声时间、酶解-超声温度对鸡骨草多糖提取率的影响,采用响应面试验优化多糖提取工艺.ELISA法检测炎症细胞因子TNF-α、IL-1β、IL-6和IL-10含量.结果:鸡骨草多糖最佳提取工艺:纤维素酶加酶量2.8%、液料比21∶1(mL/g)、酶解pH 5.0、酶解-超声功率 250 W、酶解-超声时间 90 min、酶解-超声温度 49℃,提取率为13.26%.与LPS组比较,鸡骨草多糖可降低促炎细胞因子TNF-α、IL-1β、IL-6 含量,增加抗炎细胞因子IL-10含量,差异均有统计学意义(P＜0.05).结论:超声辅助酶法提取鸡骨草多糖效率高,获得的多糖具有较强的抗炎活性.
Abstract
Objecitve:On the basis of single factor experiment,response surface experiment was performed to optimize the extraction process of polysaccharide from Abrus cantoniensis Hance by ultrasound-assisted enzyme method.Its anti-inflammatory activity was analyzed by anti-inflammatory test in vitro.Methods:The effects of enzyme dosage,liquid-solid ratio,pH of enzymolysis,enzymolysis-ultrasonic power,enzymolysis-ultrasonic time,enzymolysis-ultrasonic temperature on the extraction rate of polysaccharide were investigated by single factor test.The extraction process of polysaccharide was optimized by response surface test.The contents of TNF-α,IL-1β,IL-6 and IL-10 were detected by ELISA method.Results:The optimized extraction conditions of Abrus cantoniensis Hance polysaccharide were as follows:cellulase content 2.8%,liquid-solid ratio 21∶1(mL/g),pH 5.0,enzymolysis-ultrasonic power 250 W,enzymolysis-ultrasonic temperature 49℃,enzymolysis-ultrasonic time 90 min.Under these conditions,the extraction rate of polysaccharide was 13.26%.Compared with the LPS group,the polysaccharide could decrease the contents of pro-inflammatory cytokines TNF-α,IL-1β and IL-6,and increase the contents of anti-inflammatory cytokine IL-10.Conclusion:The extraction efficiency of Abrus cantoniensis Hance polysaccharide by ultrasound-assisted enzyme method was high,and the extracted polysaccharide possessed strong anti-inflammatory activity.
基金项目
2023年全国中药特色技术传承人才培训项目(国中医药人教函202396号)
国家科技期刊平台
NSTL
维普
万方数据