青春期双酚S暴露对雌性小鼠神经行为及脑组织抗氧化水平的影响
Effects of bisphenol S exposure during adolescence on neurobehavior and anti-oxydation level of brain tissue in female mice
张晓敏 1侯雨函 2马凯龙 2张永利3
作者信息
- 1. 兰州大学第二医院特需内科(老年病科),甘肃 兰州 730030
- 2. 兰州大学第二临床医学院,甘肃 兰州 730000
- 3. 兰州市第三人民医院老年病科,甘肃 兰州 730050
- 折叠
摘要
目的 研究青春期双酚S(BPS)暴露对雌性小鼠神经行为及脑组织抗氧化水平的影响.方法 将52只4周龄SPF级雌性C57BL/6J小鼠采用随机数字表法分为对照组及BPS低、中、高剂量组4组,每组13只.给予不同剂量BPS染毒8周后,通过旷场试验和Morris水迷宫试验对小鼠进行神经行为学观察,并检测小鼠脑组织氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化物酶(CAT)的活性.结果 旷场试验结果显示,青春期BPS暴露雌性小鼠的焦虑情绪和自发活动情况与对照组比较差异均无统计学意义(P＞0.05);Morris水迷宫试验结果显示,除第5天BPS中剂量组小鼠的逃避潜伏期明显短于其他3组(P＜0.05)外,青春期暴露于BPS的雌性小鼠与对照组雌性小鼠成年后的学习和记忆功能比较差异无统计学意义(P＞0.05);青春期暴露于BPS的雌性小鼠脑组织内氧化应激指标(MDA、SOD、GSH-Px、CAT)与对照组比较差异均无统计学意义(P＞0.05).结论 青春期BPS暴露对雌性小鼠神经行为无明显影响,也未致使小鼠脑组织出现明显的氧化应激反应.
Abstract
Objective To investigate the effects of bisphenol S(BPS)exposure on neurobehavior and anti-oxy-dation level in brain tissue of adolescent female mice.Methods 52 SPF grade female C57BL/6J mice aged 4 weeks were divided into control group and BPS low,medium and high dose groups by random number table method,with 13 mice in each group.After 8 weeks of exposure to different doses of BPS,the neurobehavioral indicators of mice were observed by open field test and Morris water maze test,and the activities of malondialdehyde(MDA),su-peroxide dismutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)were detected.Results The open field test showed that the anxiety and spontaneous activity of adolescent female mice exposed to BPS had no statisti-cal significance compared with the control group(P＞0.05).The results of Morris water maze test showed that except the escape latency of the medium dose group of BPS on day 5 was significantly shorter than that in the other 3 groups(P＜0.05),there were no other significant differences in adult learning and memory function between adoles-cent female mice exposed to BPS and the control group(P＞0.05).There were no significant differences in oxidative stress indexes(MDA,SOD,GSH-Px,CAT)between adolescent female mice exposed to BPS and the control group(P＞0.05).Conclusion BPS exposure during adolescence has no significant effect on the neurobehavior of female mice,and does not cause significant oxidative stress in the brain of mice.
关键词
双酚S/青春期/雌性小鼠/氧化应激/神经行为/实验研究Key words
bisphenol S/adolescence/female mice/oxidative stress/neurobehavior/experimental research引用本文复制引用
基金项目
兰州市科技发展指导性计划项目(2019-ZD-77)
兰州大学学生创新创业行动计划项目(20210050050)
出版年
2024
国家科技期刊平台
NSTL
万方数据