Research on healing effect of wound disinfection gel on skin injury model rat skin wounds and its network pharmacological mechanism
Objective To observe the promotion effect of wound disinfection gel on skin wound healing in rats,and explore its potential mechanism by using network pharmacology.Methods 48 rats were divided into blank group,model group and drug administration group by random number table,with 16 rats in each group.Skin wound model was established in the 2 groups except the blank group.After modeling,wound disinfection gel(1.0 mg/mm2)was applied to the wound in the drug administration group,and excipients of equal volume were applied to the blank group and the model group for a total of 28 days.The residual wound rate was calculated on the 4th,7th,14th and 28th day after administration.HE staining was performed to observe the pathological changes of the skin in each group.The drug-composition-target-disease network was constructed by Cytoscape 3.7.2,the core components and targets were searched,and the molecular docking was verified by AutoDock.Results On the 4th day after administration,the wound of rats in the administration group began to form scab,and on the 7th day after administration,the wound of rats in the model group began to form scab.Compared with the blank group,the wound residual rate in the model group was significantly increased at the 4th,7th,14th and 28th days after administration,with statistical significance(P＜0.01).Compared with the model group,the wound residual rate of rats in the admi-nistration group was significantly decreased at the 7th,14th and 28th days after administration,with statistical signi-ficance(P＜0.05 or P＜0.01).The dermis and epidermis of skin tissue of rats in blank group were intact,with few inflammatory cells.The epidermal layer of rats in the model group was thickened,and there were a large number of inflammatory cells in the dermis.On the 7th,14th and 28th days after administration,the number of capillaries in the administration group increased,and the complete basal layer of the epidermis began to form.On the 4th,7th,14th and 28th days after administration,the inflammatory cells in the administration group were significantly reduced(P＜0.05 or P＜0.01).Network pharmacology results showed that the key components of wound disinfection gel to promote skin healing were quercetin,apigenin,and ursolic acid,and the key targets were PTGS2,NCOA2,and PTGS1.Molecular docking results suggested that the binding energies of ursolic acid with PTGS1,PTGS2 and NCOA2 were low and stable.Conclusion Wound disinfection gel can promote the healing of damaged skin,and its potential mechanism is related to the regulation of inflammatory factors such as PTGS1,PTGS2 and NCOA2 by ur-solic acid.
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