Experimental study on skin toxicology of Angelica sinensis skin-nourishing and freckle-removing mask
Objective To observe the skin toxicity of Angelica sinensis skin-nourishing and freckle-removing mask and evaluate its safety.Methods Acute toxicity test,long-term toxicity test,irritation test and allergy test were conducted to observe the acute toxicity reaction,long-term toxicity reaction,irritation reaction and allergy reac-tion of the skin of guinea pig or New Zealand white rabbit.Results The results of skin acute toxicity test showed that there was no significant decrease in food intake,no significant change in respiration,physical state,eyes,mental state,limb activity,fecal characteristics,and no death.Compared with the blank group,there were no significant changes in body mass of guinea pig at different time points in each treatment group(P＞0.05).Long-term skin to-xicity test results showed that compared with the blank group,there were no significant changes in blood biochemical indexes of guinea pig[white blood cells(WBC),red blood cells(RBC),hemoglobin(Hb),platelets(PLT),aspar-tate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL),urea nitrogen(BUN),creati-nine(Cr)]in the treatment group.There were no significant differences(P＞0.05).And there were no significant changes in pancreas,lung,stomach,cerebral cortex and hippocampus CA1 area of guinea pig in each treatment group.The results of skin irritation test showed that Angelica sinensis skin-nourishing and freckle-removing mask had no skin reaction and irritation on both intact and damaged skin of New Zealand white rabbit.The results of skin al-lergy test showed that compared with the blank group,the incidence of allergy and sensitization intensity of treatment group had no significant changes at each time point,and the differences were not statistically significant(P＞0.05).Conclusion Angelica sinensis skin-nourishing and freckle-removing mask has high safety without skin toxicity,irri-tation,allergy on experimental animals,and it has a good application prospect.
Angelica sinensis skin-nourishing and freckle-removing maskskin toxicityskin irritationskin al-lergyexperimental study
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