垂盆草护肝颗粒的质量标准研究
Study on quality standard of Chuipencao Hugan Granules(垂盆草护肝颗粒)
潘鑫 1秦宝福 2史学礼 3王蓉 4周光姣4
作者信息
- 1. 西北农林科技大学生命工程学院,陕西 杨凌 712199;铜陵职业技术学院医学系,安徽 铜陵 244000
- 2. 西北农林科技大学生命工程学院,陕西 杨凌 712199
- 3. 亳州市食品药品检验中心,安徽 亳州 236800
- 4. 亳州职业技术学院药学院,安徽 亳州 236800
- 折叠
摘要
目的 建立垂盆草护肝颗粒的质量标准.方法 采用薄层色谱(TLC)法定性鉴别垂盆草护肝颗粒中垂盆草、佛手、栀子、黄连药材,高效液相色谱(HPLC)法测定垂盆草护肝颗粒中五味子醇甲、栀子苷含量,色谱柱为C18柱(250.0 mm×4.6 mm,5 μm),流速1.0 mL/min,柱温30℃,进样量10 μL.五味子醇甲流动相为甲醇-水(65∶35),检测波长250 nm;栀子苷流动相为乙腈-0.2％磷酸水溶液(10∶90),检测波长238 nm.结果 TLC斑点清晰、分离好,且阴性无干扰;五味子醇甲和栀子苷分别在0.63～31.60,5.86～58.63μg范围内线性关系良好,平均加样回收率分别为98.02％,98.19％,RSD分别为1.00％,0.99％;五味子醇甲、栀子苷的含量分别为0.051,1.249 mg/g.结论 所建立的定性、定量鉴别方法准确、可靠,重复性好、专属性强,可为垂盆草护肝颗粒质量标准的制定提供依据.
Abstract
Objective To establish the quality standard of Chuipencao Hugan Granules(垂盆草护肝颗粒).Methods Thin layer chromatography(TLC)was used to qualitatively identify Sedum Sarmentosum Bunge,Fructus Citri Sarcodactylis,Gardeniae Fructus,And Coptidis Rhizoma in Chuipencao Hugan Granules.High performance liquid chromatography(HPLC)was used to determine the contents of schisandrin and jasminoidin in Chuipencao Hugan Granules.The determination was performed on a C18 column(250.0 mm×4.6 mm,5 μm)at the flow rate of 1.0 mL/min.The column temperature was 30 ℃,and the sample size was 10 μL.The mobile phase of schisandrin was methanol-water(65:35),and the detection wavelength was 250 nm.The mobile phase of jasminoidin was ace-tonitrile-0.2％phosphoric acid aqueous solution(10∶90),and the detection wavelength was 238 nm.Results The TLC spots were clear,well separated and negative without interference.The linear relationships of schisandrin and jasminoidin were good in the range of 0.63-31.60 and 5.86-58.63 μg,respectively.The average recoveries were 98.02％and 98.19％,and RSD were 1.00％and 0.99％,respectively.The contents of schisandrin and jasminoidin were 0.051 and 1.249 mg/g,respectively.Conclusion The established qualitative and quantitative identification methods are accurate,reliable,reproducible and specific,and can provide a basis for the formulation of quality standards for Chuipencao Hugan Granules.
关键词
垂盆草护肝颗粒/五味子醇甲/栀子苷/薄层色谱/高效液相色谱/含量测定Key words
Chuipencao Hugan Granule/schisandrin/jasminoidin/thin layer chromatography(TLC)/high per-formance liquid chromatography(HPLC)/content determination引用本文复制引用
基金项目
安徽省高校自然科学研究项目(KJ2019A1035)
亳州职业技术学院自然科学研究项目(YKZYB011)
亳州职业技术学院自然科学研究项目(YKZYB008)
出版年
2024
国家科技期刊平台
NSTL
万方数据