Objective To establish a method for the identification of Ephedra przewalskii Stapf.Methods The origin identification,character identification,microscopic identification and physical and chemical identification methods were used to identify the medicinal plant Ephedra przewalskii Stapf to obtain the main identification features of morphology and histology of the medicinal material.Ephedrine and pseudoephedrine were used as the index com-ponents of Ephedra przewalskii Stapf.Thin-layer chromatography(TLC)and ultra performance liquid chromatogra-phy(UPLC)were used for qualitative identification and chromatographic characteristics were analyzed.Results The stem of Ephedra przewalskii Stapf is cylindrical,often longitudinally split into elliptical mesh on the surface,and the top of the stem is extremely twisted.Membranous scale leaves are 3-lobed,seldom 2-lobed,apex sharp,re-curved;the pith is triangular;the vertical wall of epidermal cells of squamous leaf is wavy.The stomata were invagi-nated,and the top surface of the guard cells was shaped like a telephone handset.The pollen grains are oval,the petal cells are closely arranged,the fiber wall is thick,and there are fine granular crystals on it.The transverse sec-tion of the stem is wide,small calcium oxalate crystals can be seen,cambium and xylem are triangular.TLC and UPLC results showed that ephedrine and pseudoephedrine were not found in Ephedra przewalskii Stapf.Conclusion The identification method and experimental results established in this study are efficient,accurate and reliable,and can provide reference for the identification of Ephedra przewalskii Stapf.
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