Screening of gastric cancer related plasma circRNA and bioinformatics study of its parent gene expression in gastric cancer tissues
Objective To screen the plasma differentially expressed circular RNA(circRNA)genes related to gastric cancer,verify the expression of initial screening circRNA in plasma of patients with gastric cancer,and ana-lyze the expression and clinical significance of their parent genes in gastric cancer tissues by bioinformatics methods.Methods CircRNA gene chip technology was used to obtain the expression profile of circRNA in the plasma of gastric cancer patients and normal physical examination subjects,and the circRNA whose expression difference was more than 2 times between gastric cancer patients and normal physical examination subjects was selected.Candidate circRNA genes were screened by bioinformatics methods.The parent genes corresponding to the top 10 circRNAs that were differentially expressed by a factor greater than 2 between gastric cancer tissues and adjacent tissues were analyzed[testis expressed 10(TEX10),heparin sulfate proteoglycan 2(HSPG2),tubulin e and 8 complex 1(TEDC1),integrin subunit β4(ITGB4),ADP ribosylation factor GTPase activating protein 1(ARFGAP1)]in gas-tric cancer tissues.And the differential expression between gastric cancer tissues and adjacent normal tissues in pa-tients with different proliferation stages,different differentiation degrees,helicobacter pylori(Hp)infection or not,different lymph node metastasis,and different clinicopathological subtypes was analyzed.Results By analyzing the expression profiles of circRNA in the plasma of gastric cancer patients,it was found that there were significant diffe-rences in the expression levels of 566 circrRNAs in the plasma of gastric cancer patients and normal physical exami-nation subjects(P＜0.05),and the expression levels of 68 circRNAs were more than 2 times different.Through bioin-formatics analysis,20 candidate circRNA genes were finally screened.The expressions of parent genes TEX10,HSPG2,TEDC1,ITGB4 and ARFGAP1 were significantly up-regulated in gastric cancer tissues(P＜0.001).There were significant differences in expression between gastric cancer tissues and adjacent normal tissues in patients with different proliferation stages,different differentiation degrees,Hp infection or not,different lymph node metastasis,and different clinicopathological subtypes(P＜0.05 or P＜0.01 or P＜0.001).Conclusion The circRNA expression profile related to gastric cancer was successfully constructed,and 20 differentially expressed circRNA genes were initially screened out in the plasma of gastric cancer patients.The parent genes TEX10,HSPG2,TEDC1,ITGB4,and ARFGAP1 corresponded to highly expressive hsa_circ_0087773,hsa_circ_0010697,hsa_circ_0033634,hsa_circ_0045686,and hsa_circ_0061150 in plasma were also up-regulated in gastric cancer tissues,which has certain clinical value for the diagnosis and prognosis evaluation of gastric cancer.
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