Discussion on the molecular mechanism of modified Xuemaitong No.2 Granules(加味血脉通2号颗粒)against atherosclerosis based on network pharmacology
Objective To analyze the targets and signal pathways of modified Xuemaitong No.2 Granules(加味血通2号颗粒)against atherosclerosis(AS)based on network pharmacology,and to explore its potential molecu-lar mechanism.Methods The active components and targets of modified Xuemaitong No.2 Granules were screened by TCMSP,TCMID and Swiss Target Prediction servers;GeneCards and online human Mendelian inheritance(OMIM)databases were used to find AS-related targets,and the two targets were mapped after gene standardiza-tion;The"compound-target"network was constructed by Cytoscape 3.7.1 software,and the protein-protein interac-tion(PPI)network was drawn by STRING database.The gene ontology(GO)function and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed by DAVID database.Results A total of 140 active components,943 component-related targets,2157 AS disease targets,and 389 drug-disease common tar-gets were screened;GO function enrichment analysis obtained 1291 GO entries,including 943 biological process(BP)entries,98 cell composition(CC)entries,and 250 molecular function(MF)entries,mainly involved in in-flammatory response,extracellular matrix decomposition,enzyme binding,and steroid hormone receptor activity;149 signal pathways were obtained by KEGG pathway enrichment screening,mainly involving Toll-like receptor signaling pathway,cancer,tumor necrosis factor,hypoxia-inducible factor-1 signaling pathway,phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)and so on.Conclusion Modified Xuemaitong No.2 Granules may treat AS through its active components such as ursolic acid and sitosterol by regulating inflammatory response,apoptosis,oxidative stress and other pathways through multiple targets such as MAPK1,MAPK3,MAPK8,IL-6,TNF,MMP2,MMP9.
atherosclerosismodified Xuemaitong No.2 Granules(加味血脉通2号颗粒)network pharmaco-logymechanism of action
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NSTL
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