Impacts of Piwei Peiyuan Fang(脾胃培源方)on gastric mucosal inflammation and COX-2 mRNA and protein expression in chronic atrophic gastritis model rats
Objective To investigate the impact of Piwei Peiyuan Fang(脾胃培源方)on gastric mucosal in-flammation and cyclooxygenase-2(COX-2)mRNA and protein expression in rats with chronic atrophic gastritis(CAG),and to explore the potential mechanism of Piwei Peiyuan Fang in treating CAG.Methods 8 out of 54 SPF healthy male SD rats were randomly assigned to the normal group,while the remaining rats were induced with CAG models by drinking ammonia and intragastric administration of deoxycholic acid sodium solution.After 10 weeks,40 successfully modeled rats were divided into model group,Weifuchun group,as well as high-dose,medium-dose and low-dose groups of Piwei Peiyuan Fang using a random number table method,each consisting of 8 rats.Following 4 weeks of continuous intragastric administration of corresponding drugs or normal saline,body mass growth was com-pared among the groups.Pathological changes in gastric mucosa were observed under a microscope.The expression levels of COX-2 protein and mRNA in gastric tissue were detected using western blotting,immunohistochemistry and polymerase chain reaction.Results Compared with normal group,the growth rate of body mass of rats in model group decreased significantly,the difference was statistically significant(P＜0.01).Compared with model group,the growth rate of body mass of rats in each dose group of Piwei Peiyuan Fang was significantly increased,and the differences were statistically significant(P＜0.01).Compared with normal group,the expression levels of COX-2 pro-tein and mRNA in gastric tissues of rats in model group were significantly increased,with statistical significance(P＜0.01).Compared with model group,the expression levels of COX-2 protein and mRNA in gastric tissues of rats in each dose group of Piwei Peiyuan Fang were significantly decreased,and the differences were statistically signifi-cant(P＜0.01).In the model group,gastric mucosa became thin,intrinsic glands atrophied,and inflammatory cells infiltrated the whole layer.Piwei Peiyuan Fang can obviously improve and repair the gastric mucosa of rats and re-duce the damage of gastric mucosa.Conclusion Piwei Peiyuan Fang can promote the body mass growth of CAG model rats,improve and repair the gastric mucosa of rats,and the mechanism is closely related to the inhibition of COX-2 over-expression.
chronic atrophic gastritisPiwei Peiyuan Fang(脾胃培源方)gastric mucosapathological chan-gesinflammatory responsecyclooxygenase-2ratexperimental research
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