Simultaneous determination of 6 components in the seed coat of Paeonia rockii by HPLC wavelength-switching method
Objective To establish a high-performance liquid chromatography(HPLC)wavelength-switching approach for the concurrent determination of 6 components in the seed coat of Paeonia rockii.Methods The con-tents of gallic acid,oxypaeoniflorin,albiflorin,paeoniflorin,resveratrol and luteolin in the seed coat of Paeonia rockii were determined via the HPLC wavelength-switching method.The determination was conducted on a CAPCELLPAK C18 column(250.0 mm×4.6 mm,5 µm)with the mobile phase composed of acetonitrile-0.1％phosphoric acid solu-tion at a gradient elution rate of 1.0 mL/min.The detector used was a diode array detector(DAD),and the detec-tion wavelengths were set at 273,260,230,228,305,350 nm,respectively.The column temperature was set at 30 ℃,and the sample volume was 10 μL.Results The regression equations of gallic acid,oxypaeoniflorin,albi-florin,paeoniflorin,resveratrol and luteolin were as follows:Y=30 209X+193.54(r=0.9992),Y=16 415X-118.55(r=0.9993),Y=8735.8X-5072.6(r=0.9992),Y=13 658X-39 219(r=0.9999),Y=82 092X+25 114(r=0.9990),Y=43 038X+11 152(r=0.9992),within the ranges of 0.2594 to 5.1886,0.7589 to 15.1782,1.2475 to 24.9500,11.7005 to 234.0093,respectively.Good linear relationships were observed within the ranges of 3.3526 to 67.0513 and 2.7802 to 55.6032 μg.The RSDs of the precision,repeatability and stability tests were 0.7％～1.4％(n=6),0.7％～1.9％(n=6)and 0.5％～1.0％(n=8),respectively.The average recoveries were 99.87％,100.41％,99.60％,100.57％,100.29％and 99.49％,respectively,and the RSDs were 2.0％,1.9％,1.8％,1.6％,1.8％and 1.8％.Conclusion This method features simple extraction,high extraction rate,good repeatability and high accuracy,and can be utilized for the quantitative analysis of the six components in the seed coat of Paeonia rockii.
seed coat of Paeonia rockiigallic acidoxypaeoniflorinalbiflorinpaeoniflorinresveratrolluteolinHPLCcontent determination
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