Mechanism of Sangshen Yin(桑参饮)in treating pulmonary heart disease based on network pharmacology and molecular docking
Objective To investigate the mechanism of Sangshen Yin(桑参饮)in treating chronic pulmonary heart disease using network pharmacology and molecular docking technology.Methods The chemical constituents and targets of Sangshen Yin were obtained from TCMSP and BATMAN-TCM,while the disease targets of pulmonary heart disease were screened from TTD and other therapeutic drug targets.The intersection targets of the two sets were enriched using the Kyoto Encyclopedia of Genes and Genomes(KEGG).A"TCM-chemical constituents-target-path-way"network was constructed using Cytoscape 3.7.0 software,with core targets and pathways identified through net-work topology analysis.Molecular docking technology was employed to verify the interaction between the chemical composition of the core target.Results A total of 68 chemical constituents corresponding to 81 targets were ob-tained.The core targets mainly include cyclooxygenase-2(PTGS2),serine/threonine protein kinase(AKT1),estro-gen receptor β(ESR2),inducible nitric oxide synthase(NOS2),matrix metalloproteinase 9(MMP9),peroxisome proliferator-activated receptor γ(PPARG),tumor necrosis factor(TNF),etc.KEGG enrichment analysis revealed 25 pathways,with immunoglobulin E receptor signaling pathway and tumor necrosis factor signaling pathway identified as core signaling pathways for treating pulmonary heart disease with Sangshen Yin.Results from molecular docking demonstrated a strong affinity between the core target and its components.Conclusion Hederagenin,baicalein,and other components of Sangshen Yin can act on PTGS2,AKT1,ESR2,among other targets,thereby treating pulmonary heart disease by regulating inflammation,oxidative stress,energy metabolism,angiogenesis,etc.
pulmonary heart diseaseSangshen Yinnetwork pharmacologymolecular dockingmechanism of action
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