Objective To establish a method for the determination of the fingerprint and multi-component con-tent of Jiejiu Baogan Oral Liquid(解酒保肝口服液).Methods High-performance liquid chromatography(HPLC)and the similarity software of the Chinese medicine chromatographic fingerprint similarity evaluation sys-tem were employed to establish the fingerprint of 11 batches of Jiejiu Baogan Oral Liquid.Principal component ana-lysis(PCA)and partial least squares regression analysis(PLS-DA)were utilized to analyze the quality of Jiejiu Baogan Oral Liquid.The method for determining the content of the active components in Jiejiu Baogan Oral Liquid was determined and its content was measured.Results The HPLC fingerprint of the oral liquid was established,with 29 common peaks identified,and 5 active components,namely puerarin,pistil isoflavone glycoside,rutin,luteo-lin,and quercetin,were identified and detected.The similarity of the fingerprint spectra is greater than 0.95.PCA and PLS-DA identified six main components that affect the fingerprint spectra,with a cumulative variance contribu-tion rate of 92.89％.The VIP values of chromatographic peaks 24,29,22,23(luteolin),3,18,6,17,27,20,7,25,26,and 19(pistil isoflavone glycosides)are greater than 1,which can be used as quality differential components of Jiejiu Baogan Oral Liquid.The mass concentrations of puerarin,pistil isoflavone glycoside,rutin,luteolin,and quer-cetin in 11 batches of Jijiu Baogan Oral Liquid were 106.698-236.795,0.815-1.868,14.552-16.662,8.204-13.12,and 18.731-20.427 μg/mL,respectively.Conclusion The method of HPLC fingerprint combined with pattern recognition and simultaneous determination of multi-component content offers a scientific and reliable quality evaluation approach for Jiejiu Baogan Oral Liquid,which is of great significance for the establishment of quality con-trol standards for Jiejiu Baogan Oral Liquid and also provides a reference for the quality evaluation of traditional Chinese medicine compound preparations.
维普
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