Whole-genome analysis of a rare human rotavirus strain of genotype G4P[6]-I5-A8
Objective To study the whole genome characteristics and evolutionary pattern of a genotype G4P[6]strain of group A rotavirus(RVA)CZ079 in China.Methods The genomic segments of CZ079,VP7,VP4,VP6 and NSP1 were amplified by reverse transcription polymerase chain reaction(RT-PCR),and the genotyping used online RVA automatic typing tool.DNAstar 5.1 and MEGA 11.0 were used in homology and genetic evolution analyses.Results The genotype of the CZ079 strain was G4-P[6]-I5-R1-C1-M1-A8-N1-T1-E1-H1.The typing by VP6 was type I5,and type A8 by NSP1.The amino acid differences were found between CZ079 and all three vaccine strains of LLRTM,RotaTeqTM and RotarixTM in the region 7-1b within the antigenic epitopes of VP7.After degradation of VP4,the 8-1,8-2,8-3,8-4 and 5-1 regions of VP8*antigenic epitopes showed large differences.Conclusions The RVA strain CZ079 detected in China in 2022 was a rare strain of genotype G4P[6]-I5-A8.It is hypothesized that this genotype of RVA was imported to China from Vietnam and other Southeast Asian regions,and formed a new reassortment strain by reassortment with RVA strains in China.
Group A rotavirusGenotypeReassortantPhylogenetic analysisAntigenic epitopes
NETL
NSTL
万方数据
