Evaluation on sensitivity and specificity of the rapid test of influenza virus antigen
Objective To evaluate the sensitivity and specificity of a rapid test reagent of influenza virus antigen in the application for children.Methods Double nasopharyngeal swabs were collected from 361 patients with flu-like symptoms in Children's Hospital of SooChow University from December 2021 to February 2022.The rapid test of influenza virus antigen and fluorescent PCR were used to detect influenza virus.The sensitivity and specificity of antigen test was evaluated using PCR as the reference method.Results There was no statistically significant difference in overall positive detection rates,positive detection rates among children of different genders and age groups between fluorescent PCR and the rapid test of influenza virus antigen(P＞0.05).However,the sensitivity and specificity of the rapid test of influenza virus antigen was significantly lower than those of fluorescence PCR(P＜0.05).In addition,for the sensitivity and specificity in different age groups,the rapid test had higher specificity and lower sensitivity during infants(＜3 years)indicating the diagnostic value of the positive results.For children＞6 years old,the rapid test had higher sensitivity and lower specificity,indicating the diagnostic value of the negative results.Conclusions Because of the differences in sensitivity and specificity of the rapid test of influenza virus antigen in different age groups of children,the positive results were reliable for infants and pre-school children with the possibility of missing case by false negative.For school students the negative results were reliable while positive results may lead to false diagnosis.This would assist clinicians to interpret the results of the rapid test of influenza virus antigen.
InfluenzaRapid test of antigenSensitivitySpecificityChildren
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NSTL
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