摘要
目的 分析北京市2023年5-9月份发现的猴痘病毒基因组变异及遗传进化特征,为猴痘防控措施制定提供依据.方法 收集猴痘病例咽拭子、疱疹涂抹拭子和肛拭子等样本,采用扩增子测序方法对病毒基因组进行测序,对序列进行比对和变异分析,并构建系统发育树和单倍型网络图.结果 共获得181例高质量猴痘病毒基因组序列,共携带193个碱基突变位点,其中80.8％的突变位点为APOBEC3酶驱动.系统发育分析显示,病毒均属于西非株系C.1进化分支,并可分为四个不同的进化簇.单倍型分析显示,181例病毒可分为108个单倍型.结论 北京市当前流行的猴痘病毒由多个源头输入,以及本地病毒持续传播和进化构成,有必要持续开展基因组监测和研究以评估本地病毒遗传变异规律.
Abstract
Objective To analyze the genomic variations and genetic evolution characteristics of the mpox virus identified from May to September 2023 in Beijing,so as to provide the basis for development of mpox prevention and control measures.Methods Samples of throat swabs,skin lesion swabs,and anal swabs were collected from mpox cases.The amplicon sequencing method was use for viral genomes sequencing.The sequences were aligned and analyzed for variation.A phylogenetic tree and a haplotype network were constructed.Results A total of 181 high-quality genome sequences of the mpox virus were obtained and 193 nucleotide mutation sites were identified.Among the mutation sites,80.8％were driven by the APOBEC3 enzyme.Phylogenetical analysis results showed that the viruses belonged to the C.1 branch of the West African lineage and could be further divided into four different clusters.The haplotype analysis showed that 181 mpox virus genomes can be divided into 108 haplotypes.Conclusions The current circulating mpox virus in Beijing is composed of multiple sources of importation along with continuous transmission and evolution of local mpox virus strains.It is necessary to continue genomic surveillance and research to evaluate the genetic variation of local viruses.
基金项目
首都卫生发展科研专项(2022-2G-30115)
高层次公共卫生技术人才建设项目培养计划(学科骨干-02-17)
NETL
NSTL
万方数据