Development and quantification of nucleicacid reference material of SARS-CoV-2 S protein with Del 143-145 mutation
Objective To developed the pseudovirus carrying point mutation of Del143-145 in S protein of severe acute respiratory coronavirus 2(SARS-CoV-2)as a standard reference material(SRM)of nucleic acid,so as to meet the needs of SRM in public health practice.Methods The Del143-145 in S protein as the characterized mutation of omicron variant was imported into the genome of original SARS-CoV-2 which was used as the reference genome.The pseudovirus was then prepared based on a three plasmids lentiviral packaging system.The homogeneity,long-term and short-term stability were tested,and the concentration was determined by multiple laboratories.Results The SRM showed good homogeneity which was stable for more than 6 months at-40℃ and for more than 12 hours at room temperature.Based on multiple laboratories calculation,the value of SRM was 1.37±0.24×104 copies/μL.Conclusions The SRM prepared in the study had good homogeneity and stability,and is suitable for multiple fields,including dissemination of values of quantity,verificatin of instruments and quality control.
Severe acute respiratory coronavirus 2LentivirusNucleic acid testStandard reference Material
NETL
NSTL
万方数据
