Evaluation on the staining results of ultrathin sections of virus infected cells by two en bloc staining techniques
Objective To evaluate the staining results of ultrathin sections of virus-infected cells by two en bloc staining techniques,i.e.osmium-thiocarbohydrazide-osmium(OTO)and ferrocyanide-reduced-osmium-thiocarbohydrazide-osmium(ROTO).Methods The samples of cells infected by four viruses(human adenovirus type 5,herpes simplex virus type 1,human enterovirus 71 and respiratory syncytial virus)were collected and stained by two en bloc staining methods,i.e.OTO and ROTO,respectively.Conventional staining method for ultrathin sections was used as the control.The samples were observed and photographed by transmission electron microscope(TEM)and the staining results of virus samples prepared by OTO and ROTO methods were analyzed statistically.Results The virus infected cell samples by OTO and ROTO methods showed good stained results and clear structures of the viruses,and the difference comparing to samples by conventional staining was not statistically significant(P＞0.05).Conclusions Both OTO and ROTO methods can be applied as en bloc staining of virus infected cells samples and provided foundation for serial ultrothin sectioning,preparation of large-volume virus samples and for observation by EM.
Virusen block stainingUltrathin sectionTransmission electron microscope
NETL
NSTL
万方数据
