Dynamic analysis of RNA and DNA levels in dry blood spots of infants exposed to HIV-1 after antiviral therapy
Objective To analyze the post-antiviral-treatment status of seven children exposed to HIV-1 and the changes of HIV-1 RNA and DNA levels in dry blood spots,so as to investigate relevant factors affecting the treatment outcome.Methods From 2016 to 2019,seven infants exposed to HIV-1 were followed and dry blood spots were collected at various time points from delivery to 2 years after birth.The levels of HIV-1 RNA and DNA in dry blood spots were quantified respectively,and the genotypic drug resistance was analyzed.The Brigg's logarithm values of final quantification results were calculated to draw diagram of HIV-1 RNA and DNA levels at various time points of each case,to explore the reasons for the changes of HIV-1 RNA and DNA after treatment.Results For the seven exposed infants enrolled in the group,2 cases had sustained virologic suppression(SVS)for nearly 9 months,2 cases failed to reach SVS possibly due to drug resistance,and 3 cases suffered HIV-1 RNA rebounded due to treatment cessation.The HIV-1 DNA in seven exposed infants remained stable at a high level.The 5 infants who had no effective virological inhibition showed the same trend of HIV-1 RNA and DNA levels(r2=0.487,P=0.021).Conclusions High treatment failure rate of infants exposed to HIV-1 in this study indicated the necessity to strengthen prevention mother-to-infant transmission and detection of genotypic drug resistance in HIV-1 positive pregnant women.Meanwhile,enhanced compliance management of medication and antiviral therapy of higher efficiency should be implemented to exposed infants with high dynamic levels of HIV-1 RNA and DNA after antiviral treatment.
Infant exposed to HIV-1HIV-1 RNA in dry blood spotHIV-1 DNA in dry blood spotsDrug resistance
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NSTL
万方数据
