Epidemiological and clinical characteristics of rotavirus among children under five years old with diarrhea in Xicheng district of Beijing,2015-2022
Objective To analyze the etiological and clinical characteristics of group A rotavirus(RVA)among children under five years old with diarrhea in Xicheng district of Beijing from 2015 to 2022,so as to provide evidence for clinical diagnosis,vaccine development and the prevention and control of RVA.Methods From January 2015 to December 2022,stool samples from children under five years old with diarrhea were collected monthly from two hospitals in Xicheng district of Beijing.Real-time fluorescence PCR was used to detect RVA while multiple semi-nested RT-PCR was used for genotyping.The epidemiological and clinical data were analyzed by SPSS 19.0.Chi-square test was used to compare the rates,and binary non-conditional logistic regression was used to analyze the differences in clinical features.Results A total of 1 617 stool samples were collected and the overall positive rate was 13.48％(218/1 617).In each year,December to the next February was the peak time of RVA incidence while March to May was the time of the second peak.The RVA positive rate was highest in the age group of 12-23 months(20.44％,84/411).G and P genotyping was applied for 170 positive samples.G9 was dominant among G genotypes(84.12％,143/170)and P[8]was dominant among P genotypes(93.53％,159/170).G9P[8]and G8P[8]were the most common genotype combinations.The genotype of G9P[8]was dominant from 2015 to 2021 and replaced by G8P[8]in 2022.The risk of RVA positivity among children with the highest daily diarrhea frequency ≥ 5 times was 1.481(95％CI:1.113-1.972)times the risk among children with＜5 times of diarrhea.The risk of RVA positivity among children who had watery stools was 3.971(95％CI:2.958-5.331)times the risk of risk of RVA positivity among,children who did not.Conclusions RVA showed obvious seasonality with a high incidence in winter and spring.The mainly affected age group was 12-23 months old group.Since 2022,G9P[8]had been replaced by G8P[8]as the dominant genotype.Children with watery stools and the highest daily diarrhea frequency ≥5 times have a higher risk of RVA diarrhea.
Group A rotavirusChildren under five years oldGenotypeDiarrheaWatery stool
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