Objective To investigate the role of transmembrane protein 106B(TMEM106B)in supporting the entry of multiple Omicron subvariants of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Methods TMEM106B-knockout Flp-In T-Rex293 cell line(TMEM106BKO)was constructed.The pseudoviral infection systems and the syncytia formation assay based on dual fluorescent complementary system of GFP1-10 and GFP11 were established to detect the role of TMEM106B in spike protein-mediated entry and cell-cell fusion during the infections by different SARS-CoV-2 Omicron subvariants.Results The SARS-CoV-2 Omicron subvariants showed varying degrees of reduced efficiency in infecting TMEM106BKO,and TMEM106B can facilitate cell-cell fusion mediated by spike protein of various SARS-CoV-2 Omicron subvariants.Conclusions TMEM106B acted as a receptor to support the entry and cell-cell fusion of multiple Omicron subvariants of SARS-CoV-2.It could be a new idea for the treatment of SARS-CoV-2 infection.
关键词
跨膜蛋白106B/新型冠状病毒/Omicron亚变异株
Key words
Transmembrane protein 106B/Severe acute respiratory syndrome coronavirus 2/Omicron subvariantm
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