Objective:To discover the differentially expressed genes(DEGs)in villus tissue of patients with unexplained recurrent spontaneous abortion(URSA)and to analyze their functions,aiming to elucidate the mechanisms behind the onset and progression of URSA.Methods:Villus tissue samples were collected from four URSA patients and four elective termination of pregnancy(ETP)at Department of Gynecology Outpatient of the First Affiliated Hospital of Xinjiang Medical University in March 2022.RNA-seq was employed to screen for DEGs between the two groups,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses to identify the biological functions and pathways of the identified DEGs.A protein-protein interaction(PPI)network was constructed using the STRING database,and screen out the key genes of URSA.Results:RNA-seq analysis identified a total of 3 350 DEGs,of which 2 259 were upregulated genes and 1 091 were downregulated genes in the URSA group.Through PPI network analysis,the top 10 key genes related to URSA were identified,including TYROBP,CD74,GH1,GH2,STAT5A,HLA-DRA,STAT5B,HLA-DRB1,CISH,and HLA-A.Through GO and KEGG biological functional analysis,it was found that differentially expressed mRNA is involved in various immune response pathways related to white blood cell differentiation and allogeneic immune responses.Conclusions:This study utilized transcriptome sequencing technology to obtain the mRNA differential expression profile of URSA,and screened for key genes and biological pathways that may be related to URSA.Our data provide a theoretical basis for elucidating the pathogenesis and diagnostic and therapeutic targests of URSA.
维普
万方数据